Skip to main content

Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7

  • Protocol
Public Health Microbiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 268))

Abstract

Bacterial virulence factors such as toxins are often encoded by bacteriophages. Among other examples, factors encoded by phages have been described in some of the emerging or re-emerging pathogens, including the pyrogenic exotoxin A production in group A streptococci (1), the cholera toxin in Vibrio cholerae (2), or enterotoxin production in enterohemorrhagic (EHEC) strains of E. coli (3).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Johnson, L. P., Tomai, M. A., and Schlievert, P. M. (1986) Bacteriophage involvement in group A streptococcal pyrogenic exotoxin A production. J. Bacteriol. 166, 623–627.

    PubMed  CAS  Google Scholar 

  2. Waldor, M. K. and Mekalanos, J. J. (1996) Lysogenic conversion by a filamentous phage encoding cholera toxin. Science 272, 1910–1914.

    Article  PubMed  CAS  Google Scholar 

  3. O’Brien A. D., Newland, J. W., Miller, S. F., Holmes, R. K., Williams Smith, H., and Formal, S. B. (1984) Shiga like toxin-converting phages from Escherichia coli strains that cause hemorragic colitis or infantile diarrhea. Science 226, 694–696.

    Article  CAS  Google Scholar 

  4. Watarai, M., Sato, T., Kobayashi, M., et al. (1998) Identification and characterization of a newly isolated Shiga toxin-2-converting phage from Shiga toxin-producing Escherichia coli. Infect. Immun. 66, 4100–4141.

    PubMed  CAS  Google Scholar 

  5. Muniesa, M., Recktenwald, J., Bielaszewska, M., Karch, H., and Schmidt, H. (2000) Characterization of a Shiga toxin 2e-converting bacteriophage from an Escherichia coli strain of human origin. Infect. Immun. 68, 4850–4855.

    Article  PubMed  CAS  Google Scholar 

  6. Acheson, D. W. K., Reidl, J., Zhang, X., Keusch, G. T., Mekalanos, J. J., and Waldor, M. K. (1998) In vivo transduction with Shiga toxin 1 encoding phage. Infect. Immun. 66, 4496–4498.

    PubMed  CAS  Google Scholar 

  7. Schmidt, H., Bielaszewska, M., and Karch, H. (1999) Transduction of enteric Escherichia coli isolates with a derivative of Shiga-toxin 2-encoding bacteriophage φ3538 isolated from E. coli O157:H7. Appl. Environ. Microbiol. 65, 3855–3861.

    PubMed  CAS  Google Scholar 

  8. Muniesa, M. and Jofre, J. (1998) Abundance in sewage of bacteriophages that infect Escherichia coli O157:H7 and that carry the Shiga toxin 2 gene. Appl. Environ. Microbiol. 64, 2443–2448.

    PubMed  CAS  Google Scholar 

  9. Muniesa, M. and Jofre, J. (2000) Occurrence of phages infecting Escherichia coli O157:H7 carrying the Stx2 gene in sewage from different countries. FEMS Microbiol. Lett. 183, 197–200.

    Article  PubMed  CAS  Google Scholar 

  10. Muniesa, M., Lucena, F., and Jofre, J. (1999) Comparative survival of free Shiga toxin2 encoding phages and Escherichia coli strains outside the gut. Appl. Environ. Microbiol. 65, 5615–5618.

    PubMed  CAS  Google Scholar 

  11. Beutin, L., Geier, D., Zimmermann, S., Aleksic, S., Gillespi, H. A., and Whittam, T. S. (1997) Epidemiological relatedness and clonal types of natural populations of Escherichia coli strains producing Shiga toxins in separate populations of cattle and sheep. Appl. Environ. Microbiol. 63, 2175–2180.

    PubMed  CAS  Google Scholar 

  12. Sambrook, J., Fritsch, E., and Maniatis, F. (1989) Molecular Cloning, A Laboratory Manual, 2nd ed. (Nolan, C., ed.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  13. De Man, J. C. (1975) The probability of the most probable number. Eur. J. Appl. Microbiol. 1, 72–77.

    Google Scholar 

  14. Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.

    Article  PubMed  CAS  Google Scholar 

  15. Harlow, E. and Lane, D. (1988) Antibodies, a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2004 Humana Press Inc.Totowa, NJ

About this protocol

Cite this protocol

Muniesa, M., Jofre, J. (2004). Abundance in Sewage of Bacteriophages Infecting Escherichia coli O157:H7. In: Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Public Health Microbiology. Methods in Molecular Biology, vol 268. Humana Press. https://doi.org/10.1385/1-59259-766-1:079

Download citation

  • DOI: https://doi.org/10.1385/1-59259-766-1:079

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-117-2

  • Online ISBN: 978-1-59259-766-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics