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Detection of Tetracycline Resistance Genes by PCR Methods

  • Protocol
Public Health Microbiology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 268))

Abstract

Rapid, accurate, and sensitive determination of antibiotic resistance profiles of various human and animal pathogens becomes a vital prerequisite for successful therapeutic intervention in the face of the increased occurrences of drug-resistant bacterial infections. The current methods, which are dependent on cultivation of pathogens and phenotypic expression of antibiotic resistance, usually require excessive time, special microbiological equipment, and qualified personnel. However, even with all these requisites, for example, no bacteria can be grown from more than 80% of all clinical samples sent to clinical microbiology laboratories (1). Besides the cultivation limitations, the cultivation-based determination of an antibiotic resistance profile lacks the genotypic information, which is essential for understanding the epidemiology and routes of transmission of antibiotic resistance genes. These genes often reside on mobile genetic elements and can move freely between commensal and pathogenic microbiota, occurring even between taxonomically distant clinical and environmental microbiota. Therefore, development of genotyping methods for detection of antibiotic resistance genes is highly desirable for fast, accurate, and sensitive detection of antibiotic resistance genes in a broad range of pathogenic and commensal bacteria in both clinical and environmental samples.

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Author information

Authors and Affiliations

  1. Rowett Research Institute, Aberdeen, UK

    Rustam I. Aminov

  2. Invasive Weed Managment Unit, Agricultural Research Service, United States Department of Agriculture (USDA-ARS), Urbana, IL

    Joanne C. Chee-Sanford

  3. Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL

    Natalie Garrigues

  4. Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL

    Asma Mehboob & Roderick I. Mackie

Authors
  1. Rustam I. Aminov
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  2. Joanne C. Chee-Sanford
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  3. Natalie Garrigues
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  4. Asma Mehboob
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  5. Roderick I. Mackie
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Editor information

Editors and Affiliations

  1. Planta Piloto de Procesos Industriales Microbiológicos, (PROIMI)-CONICET, San Miguel de Tucumán, Argentina

    John F. T. Spencer  & Alicia L. Ragout de Spencer  & 

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© 2004 Humana Press Inc.Totowa, NJ

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Aminov, R.I., Chee-Sanford, J.C., Garrigues, N., Mehboob, A., Mackie, R.I. (2004). Detection of Tetracycline Resistance Genes by PCR Methods. In: Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Public Health Microbiology. Methods in Molecular Biology, vol 268. Humana Press. https://doi.org/10.1385/1-59259-766-1:003

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  • DOI: https://doi.org/10.1385/1-59259-766-1:003

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-117-2

  • Online ISBN: 978-1-59259-766-6

  • eBook Packages: Springer Protocols

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