Abstract
Once a cDNA sequence for one of the adrenergic receptors (ARs) is obtained, it is useful to determine the restriction endonuclease sites in the sequence, to translate the nucleotide sequence into the protein sequence, to compare the sequence with known adrenergic receptor sequences in order to verify the receptor and subtype, to submit the sequence to GenBank, and to analyze the predicted secondary structure of the receptor.
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References
Haussler, D. (1998) Computational genefinding, in Trends Guide in Bioinformatics (Brenner, S. and Lewitter, F., eds.), Elsevier Science, Cambridge, UK, pp. 12–15.
Griffin, H. G. and Griffin, A. M. (1994) Computer analysis of sequence data, in Methods in Molecular Biology, vol. 24: Computer Analysis of Sequence Data (Griffin, A. M. and Griffin, H. G., eds), Humana, Totowa, NJ, pp. 1–8.
Altschul, S. F. (1998) Fundamentals of database searching, in Trends Guide in Bioinformatics (Brenner, S. and Lewitter, F., eds.), Elsevier Science, Cambridge, UK, pp. 7–9.
Brenner, S. (1998) Practical database searching, in Trends Guide in Bioinformatics (Brenner, S. and Lewitter, F., eds.), Elsevier Science, Cambridge, UK, pp. 9–12.
Maizel, J. V. J. and Lenk, R. P. (1981) Enhanced graphic matrix analysis of nucleic acid and protein sequences. Proc. Natl. Acad. Sci. USA 78, 7665–7669.
Needleman, S. B. and Wunsch, C. D. (1970) A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol. 48, 443–453.
Smith, T. F. and Waterman, M. S. (1981) Comparison of biosequences. Annu. Rev. Biophys. Biophys. Chem. 2, 482–489.
Higgins, D. G. and Sharp, P. M. (1989) Fast and sensitive multiple sequence alignments on a microcomputer. Comput. Appl. Biosci. 5, 151–153.
Pearson, W. R. and Lipman, D. J. (1988) Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85, 2444–2448.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403–410.
Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W., et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402.
Chou, P. Y. and Fasman, G. D. (1978) Prediction of secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47, 45–147.
Engelman, D. M., Steitz, T. A., and Goldman, A. (1986) Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annu. Rev. Biophys. Biophys. Chem. 15, 321–353.
Garnier, J., Osguthorpe, D. J., and Robson, B. (1978) Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120, 97–120.
Kyte, J. and Doolittle, R. F. (1982) A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157, 105–132.
Eisenberg, D., Weiss, R. M., and Terwilliger, T. C. (1984) The hydrophobic moment detects periodicity in protein hydrophobicity. Proc. Natl. Acad. Sci. USA 81, 140–144.
Finer-Moore, J. and Stroud, R. M. (1984) Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor. Proc. Natl. Acad. Sci. USA 81, 155–159.
Emini, E. A., Hughes, J. V., Perlow, D. S., and Boger, J. (1985) Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. J. Virol. 55, 836–839.
Karplus, P. A. and Schulz, G. E. (1985) Predicition of chain flexibility in proteins. Naturwissen 72, 212,213.
Jameson, B. A. and Wolf, H. (1988) The antigenic index: a novel algorithm for predicting antigenic determinants. Comput. Appl. Biosci. 4, 181–186.
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Deupree, J.D., Scofield, M.A., Bylund, D.B. (2000). Analyses of Adrenergic Receptor Sequences. In: Machida, C.A. (eds) Adrenergic Receptor Protocols. Methods in Molecular Biology™, vol 126. Humana Press. https://doi.org/10.1385/1-59259-684-3:53
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DOI: https://doi.org/10.1385/1-59259-684-3:53
Publisher Name: Humana Press
Print ISBN: 978-0-89603-602-4
Online ISBN: 978-1-59259-684-3
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