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Isolation of Plasmids from E. coli by Boiling Lysis

  • Protocol
E. coli Plasmid Vectors

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 235))

Abstract

The boiling lysis procedure (1) is quick to perform and, therefore, especially suitable for screening large numbers of small-volume Escherichia coli cultures. It is described with different adaptations in a variety of protocol books (2,3). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction analysis.

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References

  1. Holmes, D. S. and Quigley, M. (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114, 193–197.

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  2. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

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  3. Ausubel, F. M., Brent, R., Kingston, R. E., et al. (1997) Current Protocols in Molecular Biology, Wiley, New York.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Weill Medical College of Cornell University, New York, NY

    Sabine Ehrt & Dirk Schnappinger

Authors
  1. Sabine Ehrt
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  2. Dirk Schnappinger
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Editor information

Editors and Affiliations

  1. University of California at Berkeley, School of Public Health, Berkeley, CA

    Nicola Casali

  2. Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK

    Andrew Preston

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© 2003 Humana Press Inc.

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Ehrt, S., Schnappinger, D. (2003). Isolation of Plasmids from E. coli by Boiling Lysis. In: Casali, N., Preston, A. (eds) E. coli Plasmid Vectors. Methods in Molecular Biology™, vol 235. Humana Press. https://doi.org/10.1385/1-59259-409-3:79

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  • DOI: https://doi.org/10.1385/1-59259-409-3:79

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-151-6

  • Online ISBN: 978-1-59259-409-2

  • eBook Packages: Springer Protocols

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