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Cloning in Plasmid Vectors

  • Protocol
E. coli Plasmid Vectors

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 235))

Abstract

A fundamental step in molecular biology is the cloning of a DNA fragment insert into a plasmid vector. This allows the cloned fragment to be replicated upon transformation of the recombinant molecule into a bacterial cell (see Chapters 4 and 5) so that the DNA of interest can be investigated further. Cloning is an essential part of many experiments, including library generation (see Chapter 18) and expression studies (see Chapter 29).

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References

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Author information

Authors and Affiliations

  1. Department of Medical Microbiology, St. Bartholomew’s and Royal London School of Medicine and Dentistry, London, UK

    Carey Pashley

  2. Department of Pathology and Infectious Diseases, Royal Veterinary College, London, UK

    Sharon Kendall

Authors
  1. Carey Pashley
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  2. Sharon Kendall
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Editor information

Editors and Affiliations

  1. University of California at Berkeley, School of Public Health, Berkeley, CA

    Nicola Casali

  2. Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge, UK

    Andrew Preston

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© 2003 Humana Press Inc.

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Pashley, C., Kendall, S. (2003). Cloning in Plasmid Vectors. In: Casali, N., Preston, A. (eds) E. coli Plasmid Vectors. Methods in Molecular Biology™, vol 235. Humana Press. https://doi.org/10.1385/1-59259-409-3:121

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  • DOI: https://doi.org/10.1385/1-59259-409-3:121

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-151-6

  • Online ISBN: 978-1-59259-409-2

  • eBook Packages: Springer Protocols

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