Abstract
The selection of primers for a given polymerase chain reaction (PCR) can determine the efficiency and specificity of the PCR. Although in many cases successful PCR primers have been selected with little understanding of the principles involved, PCR can often only be achieved by using primers that are designed appropriately. Here, we give general recommendations for PCR primer selection and various aspects to be considered when designing primers.
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© 2003 Humana Press Inc.
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Hyndman, D.L., Mitsuhashi, M. (2003). PCR Primer Design. In: Bartlett, J.M.S., Stirling, D. (eds) PCR Protocols. Methods in Molecular Biology™, vol 226. Humana Press. https://doi.org/10.1385/1-59259-384-4:81
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DOI: https://doi.org/10.1385/1-59259-384-4:81
Publisher Name: Humana Press
Print ISBN: 978-0-89603-642-0
Online ISBN: 978-1-59259-384-2
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