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Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR

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Mitochondrial DNA

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 197))

Abstract

The human mitochondrial genome was completely sequenced in 1981 by Anderson and co-workers (1) and consists of a closed circular supercoiled DNA molecule of 16,569 base pairs. Mammalian cells characteristically contain a few hundred to several thousand mitochondria, each with 2-10 copies of the genome. The mitochondrial genome encodes 13 polypeptides, 22 transfer RNAs (tRNAs), and 2 rRNA. The 13 polypeptides encoded by the mitochondrial DNA (mtDNA) are essential subunits of the electron transport chain (ETC) and ATP synthase; cells lacking mtDNA are completely dependent on glycolysis for survival (2).

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC

    Janine H. Santos, Bhaskar S. Mandavilli & Bennett Van Houten

Authors
  1. Janine H. Santos
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  2. Bhaskar S. Mandavilli
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  3. Bennett Van Houten
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Editor information

Editors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC

    William C. Copeland

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© 2002 Humana Press Inc.

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Santos, J.H., Mandavilli, B.S., Van Houten, B. (2002). Measuring Oxidative mtDNA Damage and Repair Using Quantitative PCR. In: Copeland, W.C. (eds) Mitochondrial DNA. Methods in Molecular Biology™, vol 197. Humana Press. https://doi.org/10.1385/1-59259-284-8:159

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  • DOI: https://doi.org/10.1385/1-59259-284-8:159

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-972-8

  • Online ISBN: 978-1-59259-284-5

  • eBook Packages: Springer Protocols

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