Abstract
Cell-cell adhesion is dynamically rearranged in various cellular processes, including cell scattering, wound healing, developmental morphogenesis, and tumor metastasis. Cadherins are Ca2+-dependent homophilic cell-cell adhesion molecules (1) and bind β-catenin or plakoglobin (also known as γ-catenin), which in turn is linked to the actin cytoskeleton via α-catenin. This linkage between cadherins and the actin cytoskeleton is required for the development of a strong adhesive state (2). However, the mechanism underlying the dynamic rearrangement in cell-cell adhesion remains unclear.
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References
Takeichi, M. (1995) Morphogenetic roles of classic cadherins. Curr. Opin. Cell Biol. 7, 619–627.
Tsukita, S., Tsukita, S., Nagafuchi, A., and Yonemura, S. (1992) Molecular linkage between cadherins and actin filaments in cell-cell adherens junctions. Curr. Opin. Cell Biol. 4, 834–839.
Kaibuchi, K., Kuroda, S., Fukata, M., and Nakagawa, M. (1999) Regulation of cadherin-mediated cell-cell adhesion by the rho family GTPases. Curr. Opin. Cell Biol. 11, 591–596.
Fukata, M., Kuroda, S., Nakagawa, M., Kawajiri, A., Itoh, N., Shoji, I., et al. (1999) Cdc42 and Rac1 regulate the interaction of IQGAP1 with β-catenin. J. Biol. Chem. 274, 26,044–26,050.
Nagafuchi, A., Ishihara, S., and Tsukita, S. (1994) The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-α-catenin fusion molecules.J. Cell Biol. 127, 235–245.
Kuroda, S., Fukata, M., Nakagawa, M., Fujii, K., Nakamura, T., Ookubo, T., et al. (1998) Role of IQGAP1, a target of the small GTPases Cdc42 and Rac1, in regulation of E-cadherin-mediated cell-cell adhesion. Science 281, 832–835.
Kawakami, A., Kitsukawa, T., Takagi, S., and Fujisawa, H. (1996) Developmentally regulated expression of a cell surface protein, neuropilin, in the mouse nervous system. J. Neurobiol. 29, 1–17.
Yonehara, S., Ishii, A., Yonehara, M., Koyasu, S., Miyajima, A., Schreurs, J., et al. (1990) Identification of a cell surface 105 kd protein (Aic-2 antigen) which binds interleukin-3. Int. Immunol. 2, 143–150.
Itoh, N., Yonehara, S., Schreurs, J., Gorman, D. M., Maruyama, K., Ishii, A., et al. (1990) Cloning of an interleukin-3 receptor gene: a member of a distinct receptor gene family. Science 247, 324–327.
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Fukata, M., Nakagawa, M., Kuroda, S., Kaibuchi, K. (2002). Effects of Rho Family GTPases on Cell-Cell Adhesion. In: Manser, E., Leung, T. (eds) GTPase Protocols. Methods in Molecular Biology™, vol 189. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-281-3:121
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DOI: https://doi.org/10.1385/1-59259-281-3:121
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-934-6
Online ISBN: 978-1-59259-281-4
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