Abstract
Changes in gene expression underlie many biological phenomena, including cellular differentiation and activation, embryonic development, and pathological processes. In recent years, much attention has been focused on the identification of genes that are differentially expressed between normal and disease states: the isolation of disease-specific genes is not only essential for our understanding of the molecular basis of pathological conditions, but may potentially highlight novel therapeutic targets.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Hubank, M. and Schatz, D. (1994) Identifying differences in mRNA expression by representational difference analysis of cDNA. Nucl. Acids. Res., 22, 5640–5648.
Tyson, K. L., Weissberg, P. L., and Shanahan, C. M. Unpublished observations.
Duguid, J. R., Rohwer, R. G., and Seed, B. (1988) Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library. Proc. Natl. Acad. Sci., 85, 5738–5742.
Duguid, J. R. and Dinauer, M. C. (1990) Library subtraction of cDNA libraries to identify differentially expressed genes in scrapie infection. Nucl. Acids Res., 18, 2789–2792.
Shanahan, C. M., Weissberg, P. L., and Metcalfe, J. C. (1993) Isolation of gene markers of differentiated and proliferating vascular smooth-muscle cells. Circ. Res., 73, 193–204.
Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science, 257, 967–971.
Velculescu, V. E., Zhang, L., Vogelstein, B., and Kinzler, K. W. (1995) Serial analysis of gene expression. Science, 270, 484–487.
Utans, U., Liang, P., Wyner, L. R., Kamovsky, M. J., and Russell, M. E. (1994) Chronic cardiac rejection: identification of five upregulated genes in transplanted hearts by differential mRNA display. Proc. Natl. Acad. Sci., 91, 6463–6467.
Hu, E., Liang, P., and Spiegelman, B. M. (1996) AdipoQ is a novel adiposespecific gene dysregulated in obesity. J. Biol. Chem., 271, 10,697–10,703.
Lee, I. J., Soh, Y. J., and Song, B.J. (1997) Molecular characterization of fetal alcohol syndrome using mRNA differential display. Biochem. Biophys. Res. Comm., 240, 309–313.
Li, Y. L. and Youssoufian, H. (1997) MxA overexpression reveals a common genetic link in four Fanconi anemia complementation groups. J. Clin. Invest. 100, 2873–2880.
Patel, I. R., Attur, M. G., Patel, R. N., Stuchin, S. A., Abagyan, R. A., Abramson, S. B., and Amin, A. R. (1998) TNFα convertase enzyme from human arthritisaffected cartilage: Isolation of cDNA by differential display, expression of the active enzyme and regulation of TNFα. J. Immunol., 160, 4570–4579.
Velculescu, V. E., Zhang, L., Zhou, W., Vogelstein, J., Basrai, M. A., Bassett, D. E., Hieter, P., Vogelstein, B., and Kinzler, W. (1997) Characterization of the yeast transcriptome. Cell, 88, 243–251.
Madden, S. L., Galella, E.A., Zhu, J. S., Bertelsen, A. H., and Beaudry, G. A. (1997) SAGE transcript profiles for p53-dependent growth regulation. Oncogene, 15, 1079–1085.
Gonzalez-Zulueta, M., Ensz, L. M., Mukhina, G., Lebovitz, R. M., Zwacka, R. M., Engelhardt, J.F., Oberley, L. W., Dawson., V. L., and Dawson, T. M. (1998) Manganese superoxide dismutase protects nNOS neurons from NMDA and nitric oxide-mediated neurotoxicity. J. Neurosci., 18, 2040–2055.
Wada, J., Kumar, A., Ota, K., Wallner, E. I., Batlle, D. C., and Kanwar, Y. S. (1997) Representational difference analysis of cDNA of genes expressed in embryonic kidney. Kidney Int., 51, 1629–1638.
O’Neill, M. J. and Sinclair, A.H. (1997) Isolation of rare transcripts by representational difference analysis. Nucl. Acids Res. 25, 2681,2682.
Don, M. and Manuelidis, L. (1996) Visualization of viral candidate cDNAs in infectious brain fractions from Creutzfeldt-Jakob disease by representational difference analysis. J. Neurovirol. 2, 240–248.
Niwa, H., Harrison, L. C., DeAizpurua, H., and Cram, D. S. (1997) Identification of pancreatic beta cell-related genes by representational difference analysis. Endocrinology 138, 1419–1426.
Gress, T. M., Wallrapp, C., Frohme, M., MullerPillasch, F., Lacher, U., Friess, H., Buchler, M., Adler, G., and Hoheisel, J. D. (1997) Identification of genes with specific expression in pancreatic cancer by cDNA representational difference analysis. Genes Chromosomes and Cancer 19, 97–103.
Tajima, Y., Tashiro, K., and Camerini, D. (1997) Cloning of human chromosome 17-specific cDNAs using representational difference analysis and human-mouse hybrid cells. Genomics 42, 353–355.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd ed., Cold Spring Harbor Laboratory Press, New York.
Gubler, U. and Hoffman, B. J. (1983) A simple and very efficient method for generating cDNA libraries. Gene 25, 263.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Tyson, K., Shanahan, C. (1999). Use of cDNA Representational Difference Analysis to Identify Disease-Specific Genes in Human Atherosclerotic Plaques. In: Baker, A.H. (eds) Vascular Disease. Methods in Molecular Medicine™, vol 30. Humana Press. https://doi.org/10.1385/1-59259-247-3:83
Download citation
DOI: https://doi.org/10.1385/1-59259-247-3:83
Publisher Name: Humana Press
Print ISBN: 978-0-89603-731-1
Online ISBN: 978-1-59259-247-0
eBook Packages: Springer Protocols