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Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation

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Embryonic Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 185))

Abstract

Embryonic stem (ES) cells, the undifferentiated cells of early embryos are established as permanent lines (1,2) and are characterized by their self-renewal capacity and the ability to retain their developmental capacity in vivo (3) and in vitro (4–6). The pluripotent properties of ES cells are the basis of gene targeting technologies used to create mutant mouse strains with inactivated genes by homologous recombination (7).

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Authors and Affiliations

  1. In Vitro Differentiation Group, Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

    Anna M. Wobus, Kaomei Guan, Huang-Tian Yang & Kenneth R. Boheler

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  1. Anna M. Wobus
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  2. Kaomei Guan
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  1. Ottawa Health Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2002 Humana Press Inc., Totowa, NJ

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Wobus, A.M., Guan, K., Yang, HT., Boheler, K.R. (2002). Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation. In: Turksen, K. (eds) Embryonic Stem Cells. Methods in Molecular Biology™, vol 185. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-241-4:127

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  • DOI: https://doi.org/10.1385/1-59259-241-4:127

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-881-3

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