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Culture of Adipose Tissue and Isolated Adipocytes

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Adipose Tissue Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 155))

Abstract

Adipose tissue (AT) function is regulated by both short-term and long-term mechanisms. Long-term adaptations to hormonal, nutritional, and developmental factors take place over a time course of hours to days. For example, chronic alterations in the hormonal milieu (analogous to those that may occur during over- or underfeeding) have long-term effects on the cellular capacity for lipogenesis and lipolysis (1–3), as well as on the production of adipocyte secretory proteins (4,5). The mechanisms underlying these metabolic adaptations may involve pre- or posttranscriptional events that change the amount of enzyme protein or the number or activity of specific receptors or transporters. Elucidation of the mechanisms of long-term adaptations requires methods that maintain the function for AT ex vivo for periods up to 1 wk.

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Author information

Authors and Affiliations

  1. Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ, USA

    Susan K. Fried

  2. Department for Nutrition, University of Tennessee, Knoxville, TN, USA

    Naima Moustaid-Moussa

Authors
  1. Susan K. Fried
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  2. Naima Moustaid-Moussa
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Editor information

Editors and Affiliations

  1. Université de Nice-Sophia Antipolis, Nice, France

    Gérard Ailhaud

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© 2001 Humana Press Inc.

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Fried, S.K., Moustaid-Moussa, N. (2001). Culture of Adipose Tissue and Isolated Adipocytes. In: Ailhaud, G. (eds) Adipose Tissue Protocols. Methods in Molecular Biology™, vol 155. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-231-7:197

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  • DOI: https://doi.org/10.1385/1-59259-231-7:197

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-747-2

  • Online ISBN: 978-1-59259-231-9

  • eBook Packages: Springer Protocols

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