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Immunoblot Analysis of Sera from Patients and Vaccinees

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Meningococcal Vaccines

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 66))

Abstract

This chapter will describe the use of the immunoblotting method for analysing antibody specificities towards meningococcal antigens in sera from vaccinees, receiving group B meningococcal outer-membrane vesicle (OMV) vaccines, or from patients, falling ill with meningococcal disease. The power of the blotting technique lies in the simultaneous identification of many immunogenic components in a sample. Within the meningococcal field, this method has been employed in analyzes of antibody specificities of sera from vaccinees (1–6), patients (7–10), and carriers (11,12) as well as for characterization of OMV vaccines (13,14). Some of these studies present various ways of quantifying the blotting results (5,7,9,11).

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Author information

Authors and Affiliations

  1. Department of Vaccinology, National Institute of Public Health, Oslo, Norway

    Elisabeth Wedege

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  1. Elisabeth Wedege
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Editor information

Editors and Affiliations

  1. BC Research Institute for Children’s and Women’s Health, Vancouver, BC, Canada

    Andrew J. Pollard MD, PhD

  2. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Oxford, UK

    Martin C.J. Maiden PhD

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© 2001 Humana Press Inc., Totowa, NJ

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Wedege, E. (2001). Immunoblot Analysis of Sera from Patients and Vaccinees. In: Pollard, A.J., Maiden, M.C. (eds) Meningococcal Vaccines. Methods in Molecular Medicine™, vol 66. Humana Press. https://doi.org/10.1385/1-59259-148-5:275

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  • DOI: https://doi.org/10.1385/1-59259-148-5:275

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-801-1

  • Online ISBN: 978-1-59259-148-0

  • eBook Packages: Springer Protocols

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