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Isolation of M. tuberculosis RNA from Sputum

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Antibiotic Resistence

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 48))

Abstract

Analysis of RNA has many applications and has become increasingly important both in basic research and clinical application. The type and number of particular mRNA transcripts expressed in M. tuberculosis (MTB) bacilli under various conditions can provide insight into models of pathogenesis. MTB mRNA has also been demonstrated to correspond to viability in drug-treated MTB culture systems (1), thus providing an alternative to the lengthy and often difficult bacterial viability assays used in determining drug susceptibility. Isolation of MTB RNA has an immediate application in evaluation of treatment efficacy in patients. Recent studies on smear-positive pulmonary tuberculosis patients undergoing treatment with an optimal chemotherapeutic

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Author information

Authors and Affiliations

  1. VAMC Research Service, Iowa City, IA

    Lucy E. DesJardin

Authors
  1. Lucy E. DesJardin
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Editor information

Editors and Affiliations

  1. Department of Medical Microbiology, Royal Free and University College Medical School, University College London, London, UK

    Stephen H. Gillespie

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© 2001 The Humana Press Inc., Totowa, NJ

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DesJardin, L.E. (2001). Isolation of M. tuberculosis RNA from Sputum. In: Gillespie, S.H. (eds) Antibiotic Resistence. Methods in Molecular Medicine™, vol 48. Humana Press. https://doi.org/10.1385/1-59259-077-2:133

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  • DOI: https://doi.org/10.1385/1-59259-077-2:133

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-777-9

  • Online ISBN: 978-1-59259-077-3

  • eBook Packages: Springer Protocols

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