Abstract
Detection of unknown mutations and polymorphisms is a central ingredient in disease gene discovery and genetic diagnosis in addition to polymorphism discovery and genotyping programs. An optimum system for detection of sequence variation needs to offer specificity, sensitivity, flexibility, as well as ease of use.
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References
Vaughan P. and McCarthy, T.V. (1999) Glycosylase mediated polymorphism detection (GMPD): a novel process for genetic analysis. Genet. Anal. Biomol. Eng. 14, 169–175.
Lindahl, T. and Andersson, A. (1972) Rate of chain breakage at apurunic sites in double stranded deoxyribonucleic acid. Biochemistry 11, 3618–3623.
Price, A. and Lindahl, T. (1991) Enzymatic release of 5′-terminal deoxyribose phosphate residues from damaged DNA in human cells. Biochemistry 30, 8631–8637.
Lindahl, T., Ljungquist, S., Siegert, W., Nyberg, B., and Sperens, B. (1977) DNA N-glycosidases, properties of uracil-DNA glycosylase from Escherichia coli. J. Biol. Chem. 252, 3286–3294.
Duncan, B. (1981) in The Enzymes (Boyer, P., ed.), vol. 14, Academic, Orlando, FL, pp. 565–586.
Orita, M., Iwahana, H., Kanazawa, H., et al. (1989) Detection of polymorphisms of human DNA by gel electrophoresis as single strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86, 2766–2770.
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© 2000 Humana Press Inc.
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Vaughan, P. (2000). Use of Uracil DNA Glycosylase in Scanning for Unknown DNA Mutations and Polymorphisms. In: Vaughan, P. (eds) DNA Repair Protocols. Methods in Molecular Biology™, vol 152. Humana Press. https://doi.org/10.1385/1-59259-068-3:179
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DOI: https://doi.org/10.1385/1-59259-068-3:179
Publisher Name: Humana Press
Print ISBN: 978-0-89603-643-7
Online ISBN: 978-1-59259-068-1
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