Skip to main content

Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning

  • Protocol
Aqueous Two-Phase Systems: Methods and Protocols

Part of the book series: Methods in Biotechnologyâ„¢ ((MIBT,volume 11))

Abstract

Cyanobacteria, as gram-negative bacteria, are surrounded by a cell envelope consisting of the outer membrane and the plasma membrane with a peptidoglycan layer in between. However, cyanobacteria, is a unique prokaryote, because in addition to the cell envelope, there is an inner cytoplasmic membrane system-namely the chlorophyll-containing thylakoid membranes-which perform oxygenic photosynthesis, the most fundamental life process on the earth (1). This organism is also interesting from the evolutionary point of view, because it arose in a very ancient age and has survived in various environments. Furthermore, chloroplasts are believed to have evolved from cyanobacterial ancestors that developed an endosymbiotic relationship with an eukaryotic host cell.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Stanier, R. Y. and Cohen-Bazire, G. (1977) Phototropic prokaryotes: The cyanobacteria. Ann. Rev. Microbiol. 31, 225–274.

    Article  CAS  Google Scholar 

  2. Omata, T. and Murata, N. (1984) Isolation and characterization of three types of membranes from the cyanobacterium (blue-green alga) Synechocystis PCC 6714. Arch. Microbiol. 139, 113–116.

    Article  CAS  Google Scholar 

  3. Murata, N. and Omata, T. (1988) Isolation of cyanobacterial plasma membranes. Methods Enzymol. 167, 245–251.

    Article  CAS  Google Scholar 

  4. Peschek, G., Molitor, V., Tranka, M., Wastyn, M., and Erber, W. (1988) Charac-terization of cytochrome-c oxidase in isolated and purified plasma and thylakoid membranes from cyanobacteria. Methods Enzymol. 167, 437–449.

    Article  CAS  Google Scholar 

  5. Norling, B., Zarka, A., and Boussiba, B. (1997) Isolation and characterization of plasma membranes from cyanobacteria. Physiol. Plant. 99, 495–504.

    Article  CAS  Google Scholar 

  6. Jürgens, U. J. and Weckesser, J. (1988) Carotenoid-containing outer membranes of Synechocystis sp. strain PCC 6714. J. Bacteriol. 164, 384–389.

    Google Scholar 

  7. Norling, B., Mirzakhanian, V., Nilsson, F., Morré, D. J., and Andersson, B. (1994) Subfractional analysis of cyanobacterial membranes and isolation of plasma membranes by aqueous polymer two-phase partitioning. Anal. Biochem. 218, 103–111.

    Article  CAS  Google Scholar 

  8. Norling, B. Zak, E., Andersson, B., and Pakrasi, H. (1998) 2D-isolation of pure plasma and thylakoid membranes for Synechocystis sp. strain PCC6803. FEBS Lett. 436, 189–192.

    Article  CAS  Google Scholar 

  9. Rippka, R., Derulles, J., Waterbury, J. B., Herdman, M., and Stainer, R. Y. (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria.J. Gen. Microbiol. 111, 1–61.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Humana Press Inc.

About this protocol

Cite this protocol

Norling, B. (2000). Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning. In: Hatti-Kaul, R. (eds) Aqueous Two-Phase Systems: Methods and Protocols. Methods in Biotechnologyâ„¢, vol 11. Humana Press. https://doi.org/10.1385/1-59259-028-4:185

Download citation

  • DOI: https://doi.org/10.1385/1-59259-028-4:185

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-541-6

  • Online ISBN: 978-1-59259-028-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics