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Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity

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Aqueous Two-Phase Systems: Methods and Protocols

Part of the book series: Methods in Biotechnology™ ((MIBT,volume 11))

Abstract

Aqueous two-phase systems form when an aqueous solution exceeds specific

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References

  1. Albertsson, P.-Å. (1958) Partition of proteins in liquid polymer-polymer twophase systems. Nature 182, 709–711.

    Article  CAS  Google Scholar 

  2. Albertsson, P.-Å. (1986) Partition of Cell Particles andMacromolecules. Wiley, New York.

    Google Scholar 

  3. Forciniti, D., Hall, C. K., and Kula, M.-R. (1991) Influence of polymer molecular weight and temperature on phase composition in aqueous two-phase systems. Fluid Phase Equil. 61, 243–262.

    Article  CAS  Google Scholar 

  4. Albertsson, P.-Å., Sasakawa, S., and Walter, H. (1970) Cross partition and isoelectric points of proteins. Nature 228, 1329–1330.

    Article  CAS  Google Scholar 

  5. Walter, H., Sasakawa, S., and Albertsson, P.-Å. (1972) Cross-partition of proteins. Effect of ionic composition and concentration. Biochem. 11, 3880–3883.

    Article  CAS  Google Scholar 

  6. DeLigny, C. L. and Gelsema, W. J. (1982) On the influence of pH and salt composition on the partition of polyelectrolytes in aqueous two-phase systems. Separ. Sci. Technol. 17, 375–380.

    Article  CAS  Google Scholar 

  7. Eiteman, M. A. and Gainer, J. L. (1991) Predicting partition coefficients in poly(ethylene glycol)/potassium phosphate aqueous two-phase systems. J. Chromatogr. 586, 341–346.

    Article  CAS  Google Scholar 

  8. Eiteman, M. A. and Gainer, J. L. (1991) The effect of the pH difference between phases on partitioning in poly(ethylene glycol)/phosphate aqueous two phase systems. Chem. Eng. Commun. 105, 171–184.

    Article  CAS  Google Scholar 

  9. Eiteman, M. A. (1994) Partitioning of charged solutes in poly(ethylene glycol)/ potassium phosphate aqueous two-phase systems. Separ. Sci. Technol. 29, 685–700.

    Article  CAS  Google Scholar 

  10. Eiteman, M. A. (1994) Predicting partition coefficients of multi-charged solutes in aqueous two-phase systems. J. Chromatogr. A 668, 21–30.

    Article  CAS  Google Scholar 

  11. Bamberger, S., Seaman, G. V. F., Brown, J. A., and Brooks, D. E. (1984) The partition of sodium phosphate and sodium chloride in aqueous dextran poly(ethylene glycol) two-phase systems. J. Colloid Inter. Sci. 99, 187–193.

    Article  CAS  Google Scholar 

  12. Zaslavsky, B. Yu., Miheeva, L. M., Gasanova, G. Z., and Mahmudov, A. U. (1987) Influence of inorganic electrolytes on partitioning on non-ionic solutes in an aqueous dextran-poly(ethylene glycol) biphasic system. J. Chromatogr. 392, 95–100.

    Article  Google Scholar 

  13. Cascone, O., Andrews, B. A., and Asenjo, J. A. (1991) Partitioning of purification of thaumatin in aqueous two-phase systems. Enzyme Microbiol. Technol. 13,.

    Google Scholar 

  14. Johansson, G. (1971) Effects of different ions on the partitioning of proteins in an aqueous dextran-poly(ethylene glycol) two-phase system. Proc. Int. Solvent Extraction Conf., Soc. Chem. Ind., The Hague, 2, 928–935.

    CAS  Google Scholar 

  15. Schmidt, A. S., Ventom, A. M., and Asenjo, J. A. (1994) Partitioning and purification of alpha-amylase in aqueous two-phase systems. Enzyme Microbiol. Technol. 16, 131–141.

    Article  CAS  Google Scholar 

  16. Lee, C.-K. and Sandler, S. I. (1990) Vancomycin partitioning in aqueous two-phase systems: Effects of pH, salts, and an affinity ligand. Biotechnol. Bioeng. 35, 408–416.

    Article  CAS  Google Scholar 

  17. Albertsson, P.-Å., Cajarville, A., Brooks, D. E., and Tjerneld, F. (1987) Partition of proteins in aqueous polymer two-phase systems and the effect of molecular weight of the polymer. Biochim. Biophys. Acta. 926, 87–93.

    Article  CAS  Google Scholar 

  18. Forciniti, D., Hall, C. K., and Kula, M.-R. (1991) Protein partitioning at the isoelectric point: Influence of polymer molecular weight and concentration and protein size. Biotechnol. Bioeng. 38, 986–994.

    Article  CAS  Google Scholar 

  19. Cabezas, Jr., H., Evans, J. D., and Szlag, D. C. (1989) A statistical mechanical model of aqueous two-phase systems. Fluid Phase Equil. 53, 453–462.

    Article  CAS  Google Scholar 

  20. Cabezas, Jr., H., Kabiri-Badr, M., and Szlag, D. C. (1990) Statistical thermodynamics of phase separation and ion partitioning in aqueous two-phase systems. Bioseparation 1, 227–233.

    CAS  Google Scholar 

  21. Cabezas, Jr., H., Evans, J. D., and Szlag, D. C. (1990) Statistical thermodynamics of aqueous two-phase systems, in Downstream Processing and Bioseparation, ACS Symp. Ser. 419 (Hamel, J.-F. P., Hunter, J. B., and Sidkar, S. K., eds.), American Chemical Society, Washington, DC, pp. 38–52.

    Chapter  Google Scholar 

  22. Baskir, J. N., Hatton, T. A., and Suter, U. W. (1987) Thermodynamics of the separation of biomaterials in two-phase aqueous polymer systems: effects of the phase-forming polymers. Macromolecules 20, 1300–1311.

    Article  CAS  Google Scholar 

  23. Kang, C. H. and Sandler, S. I. (1987) Phase behavior of aqueous two-polymer systems. Fluid Phase Equil. 38, 245–272.

    Article  CAS  Google Scholar 

  24. Kang, C. H. and Sandler, S. I. (1988) A thermodynamic model for two-phase aqueous polymer systems. Biotechnol. Bioeng. 32, 1158–1164.

    Article  CAS  Google Scholar 

  25. King, R. S., Blanch, H. W., and Prausnitz, J. M. (1988) Molecular thermodynamics of aqueous two-phase systems for bioseparations. AIChE J. 34, 1585–1594.

    Article  CAS  Google Scholar 

  26. Haynes, C. A., Blanch, H. W., and Prausnitz, J. M. (1991) Separation of protein mixtures by extraction: thermodynamic properties of aqueous two-phase polymer systems containing salts and proteins. Fluid Phase Equil. 52, 463–474.

    Google Scholar 

  27. Forciniti, D. and Hall, C. K. (1990) Theoretical treatment of aqueous two-phase extraction by using virial expansions, in Downstream Processing and Bioseparation, ACS Symp. Ser. 419 (Hamel, J.-F. P., Hunter, J. B., and Sidkar, S. K., eds.), American Chemical Society, Washington, DC, pp. 53–68.

    Chapter  Google Scholar 

  28. Abbott, N. L., Blankschtein, D., and Hatton, T. A. (1991) Protein partitioning in two-phase aqueous polymer systems. 1. Novel physical pictures and scaling-thermodynamic formulation. Macromolecules 24, 4334–4341.

    Article  CAS  Google Scholar 

  29. Guan, Y., Treffry, T. E., and Lilley, T. H. (1994) Application of a statistical geo-metrical theory to aqueous two-phase systems. J. Chromatogr. A 668, 31–45.

    Article  CAS  Google Scholar 

  30. Haynes, C. A., Benitez, F. J., Blanch, H. W., and Prausnitz, J. M. (1993) Applica-tion of integral-equation theory to aqueous two-phase partitioning systems. AIChE J.B 1539–1557.

    Google Scholar 

  31. Baskir, J. N., Hatton, T. A., and Suter, U. W. (1989) Thermodynamics of the partitioning of biomaterials in two-phase aqueous polymer systems: Composition of lattice model to experimental data. J. Phys. Chem. 93, 2111–2122.

    Article  CAS  Google Scholar 

  32. Baughman, D. R. and Liu, Y. A. (1994) An expert network for extractive bio-separations in aqueous two-phase systems: an efficient strategy for experimental design and process development. Abstract, American Chemical Society, San Diego, CA.

    Google Scholar 

  33. Shanbhag, V. P. and Axelsson, C.-G. (1975) Hydrophobic interaction determined by partition in aqueous two-phase systems. Eur. J. Biochem. 60, 17–22.

    Article  CAS  Google Scholar 

  34. Eiteman, M. A. and Gainer, J. L. (1990) Peptide hydrophobicity and partitioning in poly(ethylene glycol)/magnesium sulfate aqueous two-phase systems. Biotechnol. Prog. 6, 479–484.

    Article  CAS  Google Scholar 

  35. Eiteman, M. A. and Gainer, J. L. (1991) A model for the prediction of partition coefficients in aqueous two-phase systems. Bioseparation 2, 31–41.

    CAS  Google Scholar 

  36. Diamond, A. D. and Hsu, J. T. (1989) Fundamental studies of biomolecule partitioning in aqueous two-phase systems. Biotechnol. Bioeng. 34, 1000–1014.

    Article  CAS  Google Scholar 

  37. Eiteman, M. A., Hassinen, C., and Veide, A. (1994) A mathematical model to predict the partition of peptides and peptide-modified proteins in aqueous two-phase systems. Biotechnol. Prog. 10, 513–519.

    Article  CAS  Google Scholar 

  38. Skoog, B. (1970) Determination of polyethylene glycols 4000 and 6000 in plasma protein preparations. Vox. Sang. 37, 345–349.

    Article  Google Scholar 

  39. Nozaki, Y. and Tanford, C. (1971) The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. J. Biol. Chem. 246, 2211–2217.

    CAS  Google Scholar 

  40. Eiteman, M. A. (1995) Hydrophobic and charge effects in the partitioning of solutes in aqueous two-phase systems, in Aqueous Biphasic Separations: Biomol-ecules to Metal Ions (Rogers, R. D. and Eiteman, M. A., eds.), Plenum, New York, pp. 31–47.

    Chapter  Google Scholar 

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Authors and Affiliations

  1. Department of Biological and Agricultural Engineering, Driftmier Engineering Center, University of Georgia, Athens, GA

    Mark A. Eiteman

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  1. Mark A. Eiteman
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Editors and Affiliations

  1. Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden

    Rajni Hatti-Kaul

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© 2000 Humana Press Inc.

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Eiteman, M.A. (2000). Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity. In: Hatti-Kaul, R. (eds) Aqueous Two-Phase Systems: Methods and Protocols. Methods in Biotechnology™, vol 11. Humana Press. https://doi.org/10.1385/1-59259-028-4:107

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  • DOI: https://doi.org/10.1385/1-59259-028-4:107

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-541-6

  • Online ISBN: 978-1-59259-028-5

  • eBook Packages: Springer Protocols

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