Abstract
Programmed cell death, or apoptosis, is an important event in the normal development and homeostasis of multicellular organisms (1). Apoptosis is regulated by a number of genes that promote or suppress cell death. The bcl-2 family of genes constitute important regulators of apoptosis. Several members of the bcl-2 family such as bcl-2 (2) and bcl-x L (3) suppress apoptosis, whereas certain other members such as bax (4), bak (5), and bik (6) promote apoptosis Certain animal viruses such as adenovirus, Epstein-Barr virus, herpesvirus simairi, and African swine fever virus also encode structural and functional homologs of Bcl-2. The methods currently available to determine the anti- or pro-apoptotic activities of various cellular and viral Bcl-2 homologs are cumbersome. Here, we describe highly reproducible, simple methods that we have developed to assay the activities of adenovirus E1B 19 kDa, Bcl-2, and various pro-apoptotic interacting partners.
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© 1999 Humana Press Inc., Totowa, NJ
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Subramanian, T., Chinnadurai, G. (1999). Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members. In: Wold, W.S.M. (eds) Adenovirus Methods and Protocols. Methods in Molecular Medicine™, vol 21. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-551-4:151
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DOI: https://doi.org/10.1385/0-89603-551-4:151
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-551-5
Online ISBN: 978-1-59259-603-4
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