Abstract
The optical activity characteristic of organic molecules is a result of the absorption of light as electrons are promoted to higher molecular orbitals. In UV-visible spectroscopy, the transition process is described by the Beer-Lambert Law, A = εcd, in which A is the experimentally measured absorbance, ε is the extinction coefficient, c is the molar concentration of solute, and d is the path length. Absorbance, A, is plotted vs energy or wavelength of the light.
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© 1997 Humana Press Inc.
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Juban, M.M., Javadpour, M.M., Barkley, M.D. (1997). Circular Dichroism Studies of Secondary Structure of Peptides. In: Shafer, W.M. (eds) Antibacterial Peptide Protocols. Methods In Molecular Biology™, vol 78. Humana Press. https://doi.org/10.1385/0-89603-408-9:73
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DOI: https://doi.org/10.1385/0-89603-408-9:73
Publisher Name: Humana Press
Print ISBN: 978-0-89603-408-2
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