Abstract
The efficiency of incorporation of fluorescein-11-dUTP with both random prime labeling of long probes (Chapter 8) and 3′-tailing of oligonucleotide probes (Chapter 11) can be estimated using the following rapid labeling assay. The basic protocol shown is for use with random prime labeled long probes, but modifications to the protocol for use with oligonucleotides are detailed in the notes. If sample identification is required, a pencil should be used to mark the membrane since some inks can interfere with the assay.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Cunningham, M. (1996). Monitoring Incorporation of Fluorescein into Nucleic Acid Probes Using a Rapid Labeling Assay. In: Harwood, A.J. (eds) Basic DNA and RNA Protocols. Methods in Molecular Biology™, vol 58. Humana Press. https://doi.org/10.1385/0-89603-402-X:65
Download citation
DOI: https://doi.org/10.1385/0-89603-402-X:65
Publisher Name: Humana Press
Print ISBN: 978-0-89603-402-0
Online ISBN: 978-1-59259-251-7
eBook Packages: Springer Protocols