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Direct Radioactive Labeling of Polymerase Chain Reaction Products

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PCR Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 15))

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Abstract

Radioactively labeled polymerase chain reaction (PCR) products are being used in an increasing number of molecular biology research techniques. Among these are PCR-based polymorphism assays such as linkage analysis (1) and detecting allelic loss in cancer cells (2). Other uses of radioactive PCR include generating probes for Southern and Northern blotting (3) and screening for polymorphisms and point mutations by the single-strand conformation polymorphism (SSCP) technique (4).

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References

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© 1993 Humana Press Inc., Totowa, NJ

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McDaniel, T., Meltzer, S.J. (1993). Direct Radioactive Labeling of Polymerase Chain Reaction Products. In: White, B.A. (eds) PCR Protocols. Methods in Molecular Biology, vol 15. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-244-2:41

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  • DOI: https://doi.org/10.1385/0-89603-244-2:41

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-244-6

  • Online ISBN: 978-1-59259-502-0

  • eBook Packages: Springer Protocols

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