Abstract
The separation of RNA on the basis of size by sucrose gradient fractionation is a technique frequently employed in a cloning strategy (1–3). After production of poly(A)+ mRNA by affinity chromatography (see Chapter 16) the RNA can be fractionated once or twice on sucrose gradients to produce a subpopulation of mRNA enriched for a particular species. The RNA fractions from the gradient can be assayed by in vitro translation and subsequent immunoprecipitation or bioassay of the products (see Chapters 19–Chapters 22).
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References
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© 1984 The Humana Press Inc.
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McGookin, R. (1984). Messenger RNA Fractionation on Neutral Sucrose Gradients. In: Walker, J.M. (eds) Nucleic Acids. Methods in Molecular Biology, vol 2. Humana Press. https://doi.org/10.1385/0-89603-064-4:121
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DOI: https://doi.org/10.1385/0-89603-064-4:121
Publisher Name: Humana Press
Print ISBN: 978-0-89603-064-0
Online ISBN: 978-1-59259-489-4
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