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High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format

  • Protocol
Molecular Diagnosis of Infectious Diseases

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 94))

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Abstract

By using automation and affinity-tag technologies, analysis of the large number of ORFs generated by genome-sequencing projects is greatly accelerated. Protocols describing culture of E. coli in automation-compatible formats and subsequent microto large-scale automated purification of 6xHis-tagged proteins are presented.

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References

  1. Schäfer, F., Römer, U., Emmerlich, M., Blümer, J., Lubenow, H., and Steinert, K. (2002) Automated high-throughput purification of 6xHis-tagged proteins. J. Biomolec. Tech. 12, 131.

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Author information

Authors and Affiliations

  1. Qiagen, Hilden, Germany

    Jutta Drees, Jason Smith, Frank Schäfer & Kerstin Steinert

Authors
  1. Jutta Drees
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  2. Jason Smith
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  3. Frank Schäfer
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  4. Kerstin Steinert
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Editor information

Editors and Affiliations

  1. Accenture GmbH, Munich, Germany

    Jochen Decler

  2. Institute for Medical Microbiology and Hygiene, University of Regensburg, Regenburg, Germany

    Udo Reischl

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© 2004 Humana Press Inc.

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Drees, J., Smith, J., Schäfer, F., Steinert, K. (2004). High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format. In: Decler, J., Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 94. Humana Press. https://doi.org/10.1385/1-59259-679-7:179

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  • DOI: https://doi.org/10.1385/1-59259-679-7:179

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-221-6

  • Online ISBN: 978-1-59259-679-9

  • eBook Packages: Springer Protocols

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