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The Use of Luciferase-Reporter Phage for Antibiotic-Susceptibility Testing of Mycobacteria

  • Protocol
Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 101))

Abstract

Prior to this decade, routine antibiotic-susceptibility testing (AST) of Mycobacterium tuberculosis clinical isolates was not advocated (1) This policy was predicated on the following assumptions:

  1. 1.

    The prevalence ofantibiotic resistance is low, occurring primarily among patients previously treated for tuberculosis (TB) who have relapsed,

  2. 2.

    Tuberculous infection progresses slowly, so that resistant strains of M tuberctlosis can be treated empirically with sequential therapeutic regimens without incurring great risks; and

  3. 3

    The long delay before AST information is available (6–12 wk) precludes a practical impact on patient care.

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Author information

Authors and Affiliations

  1. Division of Infectious Diseases, Albert Einstein College of Medicine, Bronx, NY

    Paul F. Riska & William R. Jacobs Jr.

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  1. Paul F. Riska
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  2. William R. Jacobs Jr.
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Editors and Affiliations

  1. London School of Hygiene and Tropical Medicine, London, UK

    Tanya Parish  & Neil G. Stoker  & 

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© 1998 Humana Press Inc., Totowa, NJ

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Riska, P.F., Jacobs, W.R. (1998). The Use of Luciferase-Reporter Phage for Antibiotic-Susceptibility Testing of Mycobacteria. In: Parish, T., Stoker, N.G. (eds) Mycobacteria Protocols. Methods in Molecular Biology™, vol 101. Humana Press. https://doi.org/10.1385/0-89603-471-2:431

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  • DOI: https://doi.org/10.1385/0-89603-471-2:431

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-471-6

  • Online ISBN: 978-1-59259-576-1

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