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Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms

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Patch Clamp Techniques

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

The synapse plays pivotal roles in various neuronal functions, with its efficacy determining the opening and closing of neuronal circuits. Among the parameters that determine synaptic efficacy, presynaptic factors are of crucial importance. However, these factors are among those that are the least well studied. Patch-clamp recording from the calyx of Held presynaptic terminal, described in this chapter, has enabled us to address directly questions about the presynaptic regulatory mechanisms in synaptic transmission.

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Acknowledgments

We thank Ervin Johnson for helpful comments and English-language editing.

Author information

Author notes

  1. Takayuki Yamashita

    Present address: Okinawa Institute of Science and Technology, Kunigami, Okinawa, Japan

Authors and Affiliations

  1. Okinawa Institute of Science and Technology, Kunigami, Okinawa, Japan

    Tomoyuki Takahashi, Tetsuya Hori & Yukihiro Nakamura

  2. Department of Neurophysiology, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan

    Tomoyuki Takahashi, Tetsuya Hori & Yukihiro Nakamura

  3. Laboratory of Sensory Processing, Brain Mind Institute, Faculty of Life Science, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland

    Takayuki Yamashita

Authors
  1. Tomoyuki Takahashi
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  2. Tetsuya Hori
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  3. Yukihiro Nakamura
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  4. Takayuki Yamashita
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Corresponding author

Correspondence to Tomoyuki Takahashi .

Editor information

Editors and Affiliations

  1. National Institute for Physiological Sciences National Institutes of Natural Sciences, Okazaki, Aichi, Japan

    Yasunobu Okada

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© 2012 Springer

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Takahashi, T., Hori, T., Nakamura, Y., Yamashita, T. (2012). Patch-Clamp Recording Method in Slices for Studying Presynaptic Mechanisms. In: Okada, Y. (eds) Patch Clamp Techniques. Springer Protocols Handbooks. Springer, Tokyo. https://doi.org/10.1007/978-4-431-53993-3_8

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  • DOI: https://doi.org/10.1007/978-4-431-53993-3_8

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-53992-6

  • Online ISBN: 978-4-431-53993-3

  • eBook Packages: Springer Protocols

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