Abstract
Monoclonal antibodies (MAbs) produced by a single clone of cells with homogeneous binding specificity for an antigenic determinant have been used in diagnostics and therapeutics. Many new methods have been devised by scientists for making hybridomas and MAbs. The three major steps for producing MAbs are immunization, immortalization, and isolation. Here, we describe technical details of the three important steps for generating mouse hybridomas and MAbs.
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Acknowledgment
We thank Tommy Kim for editorial assistance and Nakita Devenish for her drawing.
Disclaimer: The views expressed in this manuscript are those of the authors and do not reflect the official policy of the Department of the Army, the Department of Defense, the United States Government or the Henry M Jackson Foundation for the Advancement of Military Medicine.
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Kim, HY., Stojadinovic, A., Izadjoo, M.J. (2014). Immunization, Hybridoma Generation, and Selection for Monoclonal Antibody Production. In: Ossipow, V., Fischer, N. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 1131. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-992-5_3
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DOI: https://doi.org/10.1007/978-1-62703-992-5_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-991-8
Online ISBN: 978-1-62703-992-5
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