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Nuclear Ribosomal RNA Genes: ITS Region

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Molecular Plant Taxonomy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1115))

Abstract

Despite possible drawbacks (intraspecific polymorphisms and possible fungal contamination), sequencing of the ribosomal RNA gene ITS region remains one of the most popular nuclear sequences used for plant taxonomy and phylogeny. A protocol for PCR amplification and sequencing of this region using universal plant primers is provided.

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Acknowledgements

Denis Da Silva and Michel Grisoni (UMR PVBMT Cirad-Université de la Réunion) are acknowledged for their help in the ITS amplification protocol design.

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Authors and Affiliations

  1. UMR C53 PVBMT Université de la Réunion – Cirad, Université de la Réunion, Ile de la Réunion, France

    Pascale Besse

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  1. Pascale Besse
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Editors and Affiliations

  1. UMR C53 PVBMT Université de la Réunion-Cirad, Université de la Réunion, Ile de la Réunion, France

    Pascale Besse

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© 2014 Springer New York

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Besse, P. (2014). Nuclear Ribosomal RNA Genes: ITS Region. In: Besse, P. (eds) Molecular Plant Taxonomy. Methods in Molecular Biology, vol 1115. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-767-9_7

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  • DOI: https://doi.org/10.1007/978-1-62703-767-9_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-766-2

  • Online ISBN: 978-1-62703-767-9

  • eBook Packages: Springer Protocols

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