Abstract
Antibiotic resistance (AR), the ability of a microorganism to withstand the effects of antibiotics, is a growing and increasingly serious global public health problem. Enzymatic activation of antibiotics though the production of β-lactamase is one of the main mechanisms causing AR. Synthetic riboswitch containing aptazyme is constructed in E. coli to regulate the expression of β-lactamase through small molecule–aptamer interactions, which sharply reduces the antibiotic resistance of the engineered bacteria.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Nos. 20725308, 90913014, 20721061 and TRR61) and the Major Research Plan of China (No. 2006CB932102).
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Liu, L., Wang, S. (2014). Engineered Riboswitch as a Gene-Regulatory Platform for Reducing Antibiotic Resistance. In: Ogawa, A. (eds) Artificial Riboswitches. Methods in Molecular Biology, vol 1111. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-755-6_18
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DOI: https://doi.org/10.1007/978-1-62703-755-6_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-754-9
Online ISBN: 978-1-62703-755-6
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