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High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidics

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Animal Cell Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1104))

Abstract

The development of mammalian cell cycle synchronization techniques has greatly advanced our understanding of many cellular regulatory events and mechanisms specific to different phases of the cell cycle. In this chapter, we describe a high-throughput microfluidic-based approach for cell cycle synchronization. By exploiting the relationship between cell size and its phase in the cell cycle, large numbers of synchronized cells can be obtained by size fractionation in a spiral microfluidic channel. Protocols for the synchronization of primary cells such as mesenchymal stem cells, and immortal cell lines such as Chinese hamster ovarian cells (CHO-CD36) and HeLa cells are provided as examples.

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Author information

Authors and Affiliations

  1. NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore

    Wong Cheng Lee & Chwee Teck Lim

  2. Clearbridge BioMedics, Singapore, Singapore

    Ali Asgar S. Bhagat

  3. Department of Bioengineering, National University of Singapore, Singapore, Singapore

    Chwee Teck Lim

  4. Mechanobiology Institute, Singapore, Singapore

    Chwee Teck Lim

Authors
  1. Wong Cheng Lee
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  2. Ali Asgar S. Bhagat
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  3. Chwee Teck Lim
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Editor information

Editors and Affiliations

  1. Bioprozess- und Biosystemtechnik, Technische Universität Hamburg-Harburg, Hamburg, Germany

    Ralf Pörtner

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Lee, W.C., Bhagat, A.A.S., Lim, C.T. (2014). High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidics. In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 1104. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-733-4_1

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  • DOI: https://doi.org/10.1007/978-1-62703-733-4_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-732-7

  • Online ISBN: 978-1-62703-733-4

  • eBook Packages: Springer Protocols

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