Abstract
The recent development of High Throughput Sequencing technology has boosted the study of small regulatory RNA populations. A critical step prior to cloning and sequencing is purification of small RNA populations. Here, we report the optimization of an anion-exchange chromatography procedure in order to purify small regulatory RNAs bound on proteins. We developed this procedure to make it less time-consuming since our improved method no longer requires specific equipment and can easily be performed at the bench. We believe that our procedure will increase the robustness and accuracy of small RNA libraries in the future.
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Acknowledgments
We thank members of the Chambeyron laboratory for helpful discussion, Anna Warnet for advices, A. Pelisson and N. Lamb for reading the manuscript, M.C. Siomi and J. Brennecke for antibodies. Grentzinger was the recipient of a fellowship from French Ministère de la Recherche et de l’enseignement supérieur (MRT). Work in the Chambeyron laboratory is supported by grants from l’Association pour la Recherche sur le Cancer (ARC JR/AD/DMV-09/2/5007), ANR Jeunes chercheurs (ESSOR-JCJC-1604 01), and CNRS.
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Grentzinger, T., Chambeyron, S. (2014). Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries. In: Siomi, M. (eds) PIWI-Interacting RNAs. Methods in Molecular Biology, vol 1093. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-694-8_14
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DOI: https://doi.org/10.1007/978-1-62703-694-8_14
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Publisher Name: Humana Press, Totowa, NJ
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