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Proteome Analysis of Adenovirus Using Mass Spectrometry

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Adenovirus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1089))

Abstract

Analysis of proteins and their posttranslational modifications is important for understanding different biological events. For analysis of viral proteomes, an optimal protocol includes production of a highly purified virus that can be investigated with a high-resolving analytical method. In this Methods in Molecular Biology paper we describe a working strategy for how structural proteins in the Adenovirus particle can be studied using liquid chromatography–high-resolving mass spectrometry. This method provides information on the chemical composition of the virus particle. Further, knowledge about amino acids carrying modifications that could be essential for any part of the virus life cycle is collected. We describe in detail alternatives available for preparation of virus for proteome analysis as well as choice of mass spectrometric instrumentation suitable for this kind of analysis.

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Acknowledgement

This work was supported by the P.O. Zetterling Foundation (SBL), the Swedish Cancer Society (SBL), and the Kjell and Märta Beijer Foundation (UP). The authors wish to thank Lioudmila Elfineh for valuable discussions.

Author information

Authors and Affiliations

  1. Department of Immunology, Genetics, and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden

    Sara Bergström Lind & Ulf Pettersson

  2. Department of Chemistry-BMC, Uppsala University, Uppsala, Sweden

    Konstantin A. Artemenko

Authors
  1. Sara Bergström Lind
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  2. Konstantin A. Artemenko
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  3. Ulf Pettersson
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Editor information

Editors and Affiliations

  1. Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain

    Miguel Chillón

  2. Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain

    Assumpció Bosch

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Lind, S.B., Artemenko, K.A., Pettersson, U. (2014). Proteome Analysis of Adenovirus Using Mass Spectrometry. In: Chillón, M., Bosch, A. (eds) Adenovirus. Methods in Molecular Biology, vol 1089. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-679-5_2

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  • DOI: https://doi.org/10.1007/978-1-62703-679-5_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-678-8

  • Online ISBN: 978-1-62703-679-5

  • eBook Packages: Springer Protocols

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