Abstract
Polymerase chain reaction (PCR) analysis of the 16S–23S rRNA gene internal transcribed spacer (ITS) followed by microchip gel electrophoresis was useful for identification of staphylococci and for strain delineation of Staphylococcus aureus. In the study presented in this chapter, 74 ITS patterns were demonstrated among 1,188 isolated colonies of S. aureus: 55 patterns for methicillin-susceptible S. aureus (MSSA), 4 patterns for methicillin-resistant S. aureus (MRSA), and 15 patterns for both MSSA and MRSA, highlighting the inability of ITS pattern analysis to differentiate the MSSA and MRSA strains. To overcome this problem, simultaneous PCR amplification of the ITS region and mecA gene was applied to isolated colonies of staphylococcus species and positive-testing blood culture bottles.
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Fujita, Si. (2014). Internal Transcribed Spacer (ITS)-PCR Identification of MRSA. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 1085. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-664-1_5
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DOI: https://doi.org/10.1007/978-1-62703-664-1_5
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Publisher Name: Humana Press, Totowa, NJ
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