Abstract
The continuous emergence of multidrug-resistant bacterial pathogens is a major problem in public health. Many mechanisms may be involved in such resistance in Staphylococcus aureus. Increasing data have shown that S. aureus can invade different types of host cells, which may contribute to escape from host immune defense as well as evade the toxicity of certain antibiotics. The organism produces various cell wall-associated molecules, particularly fibronectin-binding proteins, which are important for the bacterial cells to adhere to and internalize into the host cells. Thus, the expression levels of these factors affect the bacterial capacity of adhesion and invasion. In this study, we found that different human MRSA isolates possessed different abilities to adhere to and invade the epithelial cells.
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References
Lowy FD (1998) Staphylococcus aureus Infections. N Engl J Med 339:520–532
von Eiff C, Becker K, Machka K et al (2001) Nasal carriage as a source of Staphylococcus aureus bacteremia. N Engl J Med 344:11–16
Foster TJ, Höök M (1998) Surface protein adhesins of Staphylococcus aureus. Trends Microbiol 6:484–488
Novick RP (2003) Autoinduction and signal transduction in the regulation of staphylococcal virulence. Mol Microbiol 48:1429–1449
Liang X, Yu C, Sun J et al (2006) Inactivation of a two-component signal transduction system, SaeRS, eliminates adherence and attenuates virulence of Staphylococcus aureus. Infect Immun 74:4655–4665
Liang X, Ji Y (2007) Comparative analysis of staphylococcal adhesion and internalization by epithelial cells. Methods Mol Biol 391:145–151
Sinha B, Francois PP, Nusse O et al (1999) Fibronectin-binding protein acts as Staphylococcus aureus invasin via fibronectin bridging to integrin alpha5beta1. Cell Microbiol 1:101–117
Agerer F, Michel A, Ohlsen K et al (2003) Integrin-mediated invasion of Staphylococcus aureus into human cells requires Src family protein-tyrosine kinases. J Biol Chem 278:42524–42531
Wang B, Yurecko R, Dedhar S et al (2006) Integrin-linked kinase is an essential link between integrins and uptake of bacterial pathogens by epithelial cells. Cell Microbiol 8:257–266
Alexander EH, Hudson MC (2001) Factors influencing the internalization of Staphylococcus aureus and impacts on the course of infections in humans. Appl Microbiol Biotechnol 56:361–366
Liang X, Ji Y (2006) Alpha-toxin interferes with integrin-mediated adhesion and internalization of Staphylococcus aureus by epithelial cells. Cell Microbiol 8:1656–1668
Yang J, Liang X, Ji Y (2013) The mutated staphylococcal H35A α-toxin inhibits adhesion and invasion of Staphylococcus aureus and group A streptococci. Virulence 4:77–81
Acknowledgments
This work was partially supported by grant AI057451 from the National Institute of Allergy and Infectious Disease and by USDA-MAES competitive grant. We thank the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) Program for clinical MRSA isolates supported under NIAID/NIH Contract No. N01-AI-95359.
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Yang, J., Ji, Y. (2014). Investigation of Staphylococcus aureus Adhesion and Invasion of Host Cells. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 1085. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-664-1_11
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DOI: https://doi.org/10.1007/978-1-62703-664-1_11
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Publisher Name: Humana Press, Totowa, NJ
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