Abstract
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is rapidly becoming the method or choice for measuring glucocorticoids due to significant interferences in traditional immunoassays by structurally related endogenous and exogenous steroids. The technique also lends itself to the measurement of more than one analyte during an analytical run without additional sample requirements and also the flexibility to develop assays for different sample matrices. Here methods are described for measuring a selection of glucocorticoids in serum, plasma, urine, and saliva using this state-of-the-art technology, which are all applicable to the routine clinical laboratory.
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References
Holder G (2006) Measurement of glucocorticoids in biological fluids. In: Wheeler MJ, Hutchinson JSM (eds) Hormone assays in biological fluids, 1st edn. Humana Press, Totowa, NJ, pp 141–157
Owen LJ, Halsall DJ, Keevil BK (2010) Cortisol measurements in patients receiving metyrapone therapy. Ann Clin Biochem 47:573–575
Owen LJ, Keevil BG (2007) The simultaneous measurement of 4 adrenal steroids by liquid chromatography tandem mass spectrometry with on-line solid phase extraction. Clin Chem Lab Med 45:M340
Jones RL, Owen LJ, Adaway JE et al (2012) Simultaneous analysis of cortisol and cortisone in saliva using XLC-MS/MS for fully automated solid phase extraction. J Chromatogr B 881–882:42–48
Wear JE, Owen LJ, Duxbury K et al (2007) A simplified method for the measurement of urinary free cortisol with LC-MS/MS. J Chromatogr B 858:27–31
Garde AH, Hansen AM (2005) Long-term stability of salivary cortisol. Scand J Clin Lab Invest 65:433–436
Raff H (2012) Cushing’s syndrome. Diagnosis and surveillance using salivary cortisol. Pituitary 15(1):64–70
Perogamvros I, Owen LJ, Newell-Price J et al (2009) Simultaneous measurement of cortisol and cortisone in human saliva using liquid chromatography-tandem mass spectrometry: application in basal and stimulated conditions. J Chromatogr B 877:3771–3775
Nieman LK, Biller BM, Findling JW et al (2008) The diagnosis of Cushing’s syndrome: an endocrine society clinical practice guideline. J Clin Endocrinol Metab 93:1526–1540
Persichilli S, Gervasoni J, Iavarone F et al (2010) A simple liquid chromatography-tandem mass spectrometry method for urinary free cortisol analysis: suitable for routine purpose. Clin Chem Lab Med 48:1433–1437
Ionita IA, Ahklaghi F (2010) Quantification of unbound prednisolone, prednisone, cortisol and cortisone in human plasma by ultrafiltration and direct injection into liquid chromatography tandem mass spectrometry. Ann Clin Biochem 47:350–357
Owen LJ, Haslam S, Adaway JE et al (2010) A simplified liquid chromatography tandem mass spectrometry assay using on-line solid phase extraction for the quantification of cortisol in saliva and comparison with a routine DELFIA method. Ann Clin Biochem 47:131–136
Acknowledgments
I would like to acknowledge all the staff from the Biochemistry Department at the University Hospital of South Manchester where all these assays are in routine use. I would especially like to acknowledge Brian Keevil, Joanne Adaway, and Rachel Jones who developed and validated the assays.
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Owen, L. (2013). Measurement of Glucocorticoids in Biological Fluids. In: Wheeler, M. (eds) Hormone Assays in Biological Fluids. Methods in Molecular Biology, vol 1065. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-616-0_12
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DOI: https://doi.org/10.1007/978-1-62703-616-0_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-615-3
Online ISBN: 978-1-62703-616-0
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