Abstract
Presented here is a complete molecular cloning protocol consisting of a number of separate but interconnected methods such as preparation of E. coli competent cells; in vitro DNA digestion and ligation; PCR; DNA agarose gel electrophoresis and gel extraction; and screening transformants by colony PCR, analytical restriction digests, and sequencing. The method is described in a lot of details so that it can be easily followed by those with very little relevant knowledge and skills. It also contains many tips that even experienced researchers may find useful.
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Reference
Green MR, Sambrook J (2012) Molecular cloning: a laboratory manual. CSHL Press, New York
Acknowledgements
I would like to thank Julia S.P. Mawer for the critical reading of the manuscript and helpful suggestions.
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© 2013 Springer Science+Business Media, New York
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Makovets, S. (2013). Basic DNA Electrophoresis in Molecular Cloning: A Comprehensive Guide for Beginners. In: Makovets, S. (eds) DNA Electrophoresis. Methods in Molecular Biology, vol 1054. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-565-1_2
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DOI: https://doi.org/10.1007/978-1-62703-565-1_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-564-4
Online ISBN: 978-1-62703-565-1
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