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Methods to Study the Proliferation and Differentiation of Cardiac Side Population (CSP) Cells

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Cellular Cardiomyoplasty

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1036))

Abstract

Investigation of cardiac progenitor cell proliferation and differentiation is essential for both the basic understanding of progenitor cell biology as well as the development of cellular therapeutics for tissue regeneration. Herein, we describe techniques used for the analysis of CSP cell proliferation, cell cycle status, and cardiomyogenic differentiation.

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References

  1. Bergmann O, Bhardwaj RD, Bernard S et al (2009) Evidence for cardiomyocyte renewal in humans. Science 324:98–102

    Article  PubMed  CAS  Google Scholar 

  2. Beltrami A, Urbanek K, Kajstura J et al (2001) Evidence that human cardiac myocytes divide after myocardial infarction. N Engl J Med 344:1750–1757

    Article  PubMed  CAS  Google Scholar 

  3. Linke A, MΓΌller P, Nurzynska D et al (2005) Stem cells in the dog heart are self-renewing, clonogenic, and multipotent and regenerate infarcted myocardium, improving cardiac function. Proc Natl Acad Sci USA 102:8966–9037

    Article  PubMed  CAS  Google Scholar 

  4. Hsieh P, Segers V, Davis M et al (2007) Evidence from a genetic fate-mapping study that stem cells refresh adult mammalian cardiomyocytes after injury. Nat Med 13:970–974

    Article  PubMed  CAS  Google Scholar 

  5. Bolli R, Chugh A, D’Amario D et al (2011) Cardiac stem cells in patients with ischaemic cardiomyopathy (scipio): initial results of a randomised phase 1 trial. Lancet 378:1847–1904

    Article  PubMed  Google Scholar 

  6. Pfister O, Fdr M, Jain M et al (2005) Cd31- but not cd31+ cardiac side population cells exhibit functional cardiomyogenic differentiation. Circ Res 97:52–113

    Article  PubMed  CAS  Google Scholar 

  7. Pfister O, Liao R (2008) Pump to survive: novel cytoprotective strategies for cardiac progenitor cells. Circ Res 102:998–1001

    Article  PubMed  CAS  Google Scholar 

  8. Sirish P, Lopez JE, Li N et al (2012) Microrna profiling predicts a variance in the proliferative potential of cardiac progenitor cells derived from neonatal and adult murine hearts. J Mol Cell Cardiol 52:264–272

    Article  PubMed  CAS  Google Scholar 

  9. Oikonomopoulos A, Sereti KI, Conyers F et al (2011) Wnt signaling exerts an antiproliferative effect on adult cardiac progenitor cells through igfbp3. Circ Res 109:1363–1374

    Article  PubMed  CAS  Google Scholar 

  10. Sakaue-Sawano A, Kurokawa H, Morimura T et al (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132:487–498

    Article  PubMed  CAS  Google Scholar 

  11. Beltrami A, Barlucchi L, Torella D et al (2003) Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell 114:763–839

    Article  PubMed  CAS  Google Scholar 

  12. Oh H, Bradfute S, Gallardo T et al (2003) Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction. Proc Natl Acad Sci USA 100:12313–12321

    Article  PubMed  CAS  Google Scholar 

  13. Matsuura K, Nagai T, Nishigaki N et al (2004) Adult cardiac sca-1-positive cells differentiate into beating cardiomyocytes. J Biol Chem 279:11384–11475

    Article  PubMed  CAS  Google Scholar 

  14. Martin C, Meeson A, Robertson S et al (2004) Persistent expression of the atp-binding cassette transporter, abcg2, identifies cardiac sp cells in the developing and adult heart. Dev Biol 265:262–337

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This work was supported in part by NIH grants (HL086967 and HL093148) to R.L. K.-I.S. was supported by the Manasaki fellowship from the University of Crete-Greece.

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© 2013 Springer Science+Business Media New York

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Sereti, KI., Oikonomopoulos, A., Unno, K., Liao, R. (2013). Methods to Study the Proliferation and Differentiation of Cardiac Side Population (CSP) Cells. In: Kao, R. (eds) Cellular Cardiomyoplasty. Methods in Molecular Biology, vol 1036. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-511-8_9

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  • DOI: https://doi.org/10.1007/978-1-62703-511-8_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-510-1

  • Online ISBN: 978-1-62703-511-8

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