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In Vivo Testing for Gold Nanoparticle Toxicity

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NanoBiotechnology Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1026))

Abstract

A technique for measuring the toxicity of nanomaterials using a murine model is described. Blood samples are collected via submandibular bleeding while urine samples are collected on cellophane sheets. Both biosamples are then analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES) for nanotoxicity. Blood samples are further tested for immunological response using a standard Coulter counter. The major organs of interest for filtration are also digested and analyzed via ICP-OES, producing useful information regarding target specificity of the nanomaterial of interest. Collection of the biosamples and analysis afterward is detailed, and the operation of the technique is described and illustrated by analysis of the nanotoxicity of an injection of a modified tiopronin monolayer-protected cluster.

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Author information

Authors and Affiliations

  1. University of Colorado, Boulder, CO, USA

    Carrie A. Simpson

  2. University of North Alabama, Florence, AL, USA

    Brian J. Huffman

  3. Vanderbilt University, Nashville, TN, USA

    David E. Cliffel

Authors
  1. Carrie A. Simpson
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  2. Brian J. Huffman
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  3. David E. Cliffel
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Editor information

Editors and Affiliations

  1. Dept. Chemistry, Vanderbilt University, Stevenson Center 7330, Nashville, 37235, Tennessee, USA

    Sandra J. Rosenthal

  2. Dept. Chemistry, Vanderbilt University, Stevenson Center 7330, Nashville, 37235, Tennessee, USA

    David W Wright

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© 2013 Springer Science+Business Media New York

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Simpson, C.A., Huffman, B.J., Cliffel, D.E. (2013). In Vivo Testing for Gold Nanoparticle Toxicity. In: Rosenthal, S., Wright, D. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology, vol 1026. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-468-5_14

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  • DOI: https://doi.org/10.1007/978-1-62703-468-5_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-467-8

  • Online ISBN: 978-1-62703-468-5

  • eBook Packages: Springer Protocols

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