Abstract
Small interfering RNA (siRNA) has the potential to influence gene expression with a high degree of target gene specificity. However, the clinical application of siRNA therapeutics has proven to be less promising as evidenced by its poor intracellular uptake, instability in vivo, and nonspecific immune stimulations. Recently, we have demonstrated that single-walled carbon nanotube (SWNT)-mediated siRNA delivery can enhance the efficiency of siRNA-mediated gastrin-releasing peptide receptor (GRP-R) gene silencing by stabilizing siRNA while selectively targeting tumor tissues. Based on our recent findings, we introduce a novel technique to silence specific gene(s) in human neuroblastoma through SWNT-mediated siRNA delivery in vitro and in vivo.
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References
Kushner BH, Kramer K, LaQuaglia MP, Modak S, Yataghene K, Cheung NK (2004) Reduction from seven to five cycles of intensive induction chemotherapy in children with high-risk neuroblastoma. J Clin Oncol 22:4888–4892
Kim S, Hu W, Kelly DR, Hellmich MR, Evers BM, Chung DH (2002) Gastrin-releasing peptide is a growth factor for human neuroblastomas. Ann Surg 235:621–629, discussion 629–30
Moody TW, Carney DN, Cuttitta F, Quattrocchi K, Minna JD (1985) High affinity receptors for bombesin/GRP-like peptides on human small cell lung cancer. Life Sci 37:105–113
Markwalder R, Reubi JC (1999) Gastrin-releasing peptide receptors in the human prostate: relation to neoplastic transformation. Cancer Res 59:1152–1159
Qiao J, Kang J, Cree J, Evers BM, Chung DH (2005) Gastrin-releasing peptide-induced down-regulation of tumor suppressor protein PTEN (phosphatase and tensin homolog deleted on chromosome ten) in neuroblastomas. Ann Surg 241:684–691, discussion 691–692
Qiao J, Kang J, Ishola TA, Rychahou PG, Evers BM, Chung DH (2008) Gastrin-releasing peptide receptor silencing suppresses the tumorigenesis and metastatic potential of neuroblastoma. Proc Natl Acad Sci USA 105:12891–12896
Liu Z, Winters M, Holodniy M, Dai H (2007) siRNA delivery into human T cells and primary cells with carbon-nanotube transporters. Angew Chem Int Ed Engl 46:2023–2027
Kam NW, Liu Z, Dai H (2006) Carbon nanotubes as intracellular transporters for proteins and DNA: an investigation of the uptake mechanism and pathway. Angew Chem Int Ed Engl 45:577–581
Liu Z, Tabakman SM, Chen Z, and Dai H (2009) Preparation of carbon nanotube bioconjugates for biomedical applications. Nat Protoc 4:1372–1382
Schipper ML, Nakayama-Ratchford N, Davis CR, Kam NW, Chu P, Liu Z, Sun X, Dai H and Gambhir SS (2008) A pilot toxicology study of single-walled carbon nanotubes in a small sample of mice. Nat Nanotechnol 3: 216–221
Cherukuri P, Bachilo SM, Litovsky SH, Weisman RB (2004) Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells. J Am Chem Soc 126:15638–15639
Cherukuri P, Gannon CJ, Leeuw TK, Schmidt HK, Smalley RE, Curley SA, Weisman RB (2006) Mammalian pharmacokinetics of carbon nanotubes using intrinsic near-infrared fluorescence. Proc Natl Acad Sci USA 103:18882–18886
Welsher K, Liu Z, Sherlock SP, Robinson JT, Chen Z, Daranciang D, Dai H (2009) A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice. Nat Nanotechnol 4:773–780
Tucker-Schwartz JM, Hong T, Colvin DC, Xu YQ, Skala MC (2012) Dual-modality photothermal optical coherence tomography and magnetic-resonance imaging of carbon nanotubes. Opt Lett 37:872–874
Hong T, Lazarenko RM, Colvin DC, Flores RL, Zhang Q, Xu Y-Q (2012) Effect of Âcompetitive surface functionalization on Âdual-modality fluorescence and magnetic resonance imaging of single-walled Âcarbon nanotubes. J Phys Chem C 116:16319–16324
Chung DH, Kang JH, Ishola TA, Baregamian N, Mourot JM, Rychahou PG, Evers BM (2007) Bombesin induces angiogenesis and neuroblastoma growth. Cancer Lett 253:273–281
Acknowledgments
The authors would like to thank Nadja Colon and Roel L. Flores for critical review of the manuscript. This work was supported by R01 DK61470 (DHC) from the National Institutes of Health and ECCS-1055852 from National Science Foundation (YQX).
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Qiao, J., Hong, T., Guo, H., Xu, YQ., Chung, D.H. (2013). Single-Walled Carbon Nanotube-Mediated Small Interfering RNA Delivery for Gastrin-Releasing Peptide Receptor Silencing in Human Neuroblastoma. In: Rosenthal, S., Wright, D. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology, vol 1026. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-468-5_11
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DOI: https://doi.org/10.1007/978-1-62703-468-5_11
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