Abstract
The use of cultures has informed us of functions and regulation of astrocytes that were previously unknown. This chapter details the methods that result in such cultures.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ransom BR, Ransom CB (2012) Astrocytes: multitalented stars of the central nervous system. In: Milner R (ed) Methods in molecular biology 814. Human Press, New York, NY, pp 3–7
Oberheim NA, Goldman SA, Nedergaard M (2012) Heterogeneity of astrocytic form and function. In: Milner R (ed) Methods in molecular biology 814. Human Press, New York, NY, pp 23–45
Barres BA (2008) The mystery and magic of glia: a perspective on their roles in health and disease. Neuron 60:430–440
McCarthy KD, de Vellis J (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol 85:890–902
Jean YY, Lercher LD, Dreyfus CF (2008) Glutamate elicits release of BDNF from basal forebrain astrocytes in a process dependent on metabotropic receptors and the PLC pathway. Neuron Glia Biol 4:35–42
Zamanian JL, Xu L, Foo LC et al (2012) Genomic analysis of reactive astrogliosis. The J of Neuroscie 32(18):6391–6410
Casper KB, Jones K, McCarthy KD (2007) Characterization of astrocyte-specific conditional knockouts. Genesis 45:292–299
Acknowledgment
The development of this protocol was supported by NIH HD 23315.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Huang, Y., Dreyfus, C.F. (2013). Culturing Astrocytes from Postnatal Rats. In: Zhou, R., Mei, L. (eds) Neural Development. Methods in Molecular Biology, vol 1018. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-444-9_7
Download citation
DOI: https://doi.org/10.1007/978-1-62703-444-9_7
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-443-2
Online ISBN: 978-1-62703-444-9
eBook Packages: Springer Protocols