Abstract
Microsatellites, or simple sequence repeats (SSRs), have proven to be an important molecular marker in plant genetics and breeding research. The main strategies to obtain these markers can be through genomic DNA and from expressed sequence tags (ESTs) from mRNA/cDNA libraries. Genetic studies using microsatellite markers have increased rapidly because they can be highly polymorphic, codominant markers and they show heterozygous conserved sequences. Here, we describe a methodology to obtain microsatellite using the enrichment library of DNA genomic sequences. This method is highly efficient to development microsatellite markers especially in plants that do not have available ESTs or genome databases. This methodology has been used to enrich SSR marker libraries in Citrus spp., an important tool to genotype germplasm, to select zygotic hybrids, and to saturate genetic maps in breeding programs.
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Acknowledgments
The authors gratefully acknowledge the financial support by INCT Project, CNPq, and FAPESP. V.M.N., M.C.Y., M.B., and M.A.M. are recipients of research fellowships from CNPq.
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Novelli, V.M., Cristofani-Yaly, M., Bastianel, M., Palmieri, D.A., Machado, M.A. (2013). Screening of Genomic Libraries. In: Kantartzi, S. (eds) Microsatellites. Methods in Molecular Biology, vol 1006. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-389-3_2
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DOI: https://doi.org/10.1007/978-1-62703-389-3_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-388-6
Online ISBN: 978-1-62703-389-3
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