Abstract
Anaplastic large-cell lymphoma (ALCL) is characterized by molecular abnormalities involving ALK gene located at 2p23 which results in an overexpression of ALK. Nine different rearrangements of ALK gene have been reported; the t(2;5)(p23;q35) is the most common. The t(2;5) fuses the NPM gene at 5q35 with the ALK gene. NPM/ALK, as well as ALK activation via other molecular abnormalities, plays an important role in the pathogenesis of ALCL. The lack of tight clustering within the involved NPM and ALK genes precludes analysis using standard PCR methods. We describe a long-range nested PCR assay to detect NPM/ALK gene rearrangements.
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References
Delsol G, Falini B, Muller-Hermelink HK et al (2008) Anaplastic large cell lymphoma (ALCL), ALK-positive. In: Swerdlow SH, Campo E, Harris NL et al (eds) WHO classification of tumours of haematopoietic and lymphoid tissues. IARC, Lyon, pp 312–316
Medeiros LJ, Elenitoba-Johnson KS (2007) Anaplastic large cell lymphoma. Am J Clin Pathol 127:707–722
Pulford K, Morris SW, Turturro F (2004) Anaplastic lymphoma kinase proteins in growth control and cancer. J Cell Physiol 199:330–358
Medeiros LJ (2008) Anaplastic large-cell lymphoma. In: Ioachim HL, Medeiros LJ (eds) Ioachim’s lymph node pathology. Lippincott Williams & Wilkins, Philadelphia, pp 504–516
Falini B (2001) Anaplastic large cell lymphoma: pathological, molecular, and clinical features. Br J Haematol 114:741–760
Drexler HG, Gignac SM, von Wasielewski R, Werner M, Dirks WG (2000) Pathobiology of NPM-ALK and variant fusion genes in anaplastic large cell lymphoma and other lymphomas. Leukemia 14:1533–1559
Duyster J, Bai RY, Morris SW (2001) Translocations involving anaplastic lymphoma kinase (ALK). Oncogene 20:5623–5637
Kuefer MU, Look AT, Pulford K, Behm FG, Pattengale PK, Mason DY et al (1997) Retrovirus-mediated gene transfer of NPM-ALK causes lymphoid malignancy in mice. Blood 90:2901–2910
Chiarle R, Gong JZ, Guasparri I, Pesci A, Cai J, Liu J et al (2003) NPM-ALK transgenic mice spontaneously develop T-cell lymphomas and plasma cell tumors. Blood 101:1919–1927
Vega F, Medeiros LJ (2003) Chromosomal translocation involved in non-Hodgkin lymphomas. Arch Pathol Lab Med 127:1148–1160
Sarris AH, Luthra R, Cabanillas F, Morris SW, Pugh WC (1998) Genomic DNA amplification and the detection of t(2;5)(p23;q35) in lymphoid neoplasms. Leuk Lymphoma 29:507–514
Kalinova M, Krskova L, Brizova H, Kabickova E, Kepak T, Kodet R (2008) Quantitative PCR detection of NPM/ALK fusion gene and CD30 gene expression in patients with anaplastic large cell lymphoma-residual disease monitoring and a correlation with the disease status. Leuk Res 32:25–32
Luthra R, Sarris AH, Papadimitracopoulou V, Waasdorp M, Dimopoulos MA, McBride JA et al (1997) Long-range amplification of genomic DNA detects the t(2;5)(p23;q35) in anaplastic large-cell lymphoma, but not in other non-Hodgkin’s lymphomas, Hodgkin’s disease, or lymphomatoid papulosis. Ann Oncol 8(Suppl 2):59–63
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Yin, C.C., Luthra, R. (2013). Detection of t(2;5)(p23;q35) in Anaplastic Large-Cell Lymphoma by Long-Range Nested Polymerase Chain Reaction Assay. In: Czader, M. (eds) Hematological Malignancies. Methods in Molecular Biology, vol 999. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-357-2_15
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DOI: https://doi.org/10.1007/978-1-62703-357-2_15
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Publisher Name: Humana Press, Totowa, NJ
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