Abstract
Enzyme-coated magnetic bioreactor particles enable a fast, convenient approach to metabolic screening. A semi-automated metabolite-profiling technique using these particles in a 96-well plate with liquid chromatography (LC)-mass spectrometry (MS)/MS detection is described. Reactions can be investigated over 1- to 2-min periods, and 96 or more reactions or reaction time points can be processed in parallel. Incorporation of DNA in the particle films facilitates determination of rates of DNA damage and metabolite-DNA adduct structures.
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References
Bajrami B, Zhao L, Schenkman JB, Rusling JF (2009) Rapid LC-MS drug metabolite profiling using microsomal enzyme bioreactors in a parallel processing format. Anal Chem 81:9921–9929
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Zhao L, Schenkman JB, Rusling JF (2010) High-throughput metabolic toxicity screening using magnetic biocolloid reactors and LC−MS/MS. Anal Chem 82:10172–10178
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© 2013 Springer Science+Business Media New York
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Zhao, L., Bajrami, B., Rusling, J.F. (2013). Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles. In: Phillips, I., Shephard, E., Ortiz de Montellano, P. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 987. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-321-3_11
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DOI: https://doi.org/10.1007/978-1-62703-321-3_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-320-6
Online ISBN: 978-1-62703-321-3
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