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Nonradioactive In Vitro Assays for Histone Deacetylases

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Protein Acetylation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 981))

Abstract

The effect of histone deacetylases (HDACs) on normal and aberrant gene expression has been studied widely, making these enzymes interesting targets for the treatment of cancer and other diseases. In this chapter, we present in vitro assays that are commonly used to detect HDAC activity that do not rely on radioactive substrates and are amenable for high-throughput testing in microtiter plates. The major focus is on in vitro screening, but we also provide protocols to monitor HDAC activity from cancer cells and peripheral white blood cells. We will discuss the advantages and drawbacks of the respective protocols and give general hints and suggestions that are valuable to obtain reliable and reproducible results.

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Acknowledgment

Work on HDAC assays in blood cells is funded by the Deutsche Forschungsgemeinschaft (DFG, SPP1463, Ju 295/9-1).

Author information

Authors and Affiliations

  1. Institute of Pharmaceutical Sciences, University of Freiburg, Freiburg, Germany

    Alexander-Thomas Hauser, Julia M. Gajer (née Wagner) & Manfred Jung

  2. Freiburg Institute of Advanced Studies (FRIAS), University of Freiburg, Freiburg, Germany

    Manfred Jung

Authors
  1. Alexander-Thomas Hauser
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  2. Julia M. Gajer (née Wagner)
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  3. Manfred Jung
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Editor information

Editors and Affiliations

  1. Adolf-Butenandt-Institut, Department of Molecular Biology, Ludwig-Maximilians-Universität München, Schillerstr. 44, München, 80336, Germany

    Sandra B. Hake

  2. , Dep. of Cell & Developmental Biology, Biocenter University of Würzburg, Am Hubland, Würzburg, 97074, Germany

    Christian J. Janzen

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Hauser, AT., Gajer (née Wagner), J.M., Jung, M. (2013). Nonradioactive In Vitro Assays for Histone Deacetylases. In: Hake, S., Janzen, C. (eds) Protein Acetylation. Methods in Molecular Biology, vol 981. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-305-3_17

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  • DOI: https://doi.org/10.1007/978-1-62703-305-3_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-304-6

  • Online ISBN: 978-1-62703-305-3

  • eBook Packages: Springer Protocols

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