Abstract
Reducing monosaccharides were derivatized with 2-aminobenzoic acid (2-AA) through reductive amination using sodium cyanoborohydride as a reductant, and the derivatives were separated by capillary zone electrophoresis with UV detection using 50 mM sodium phosphate (pH 5.5) or 150 mM sodium borate—50 mM sodium phosphate (pH 7.0) running buffer. The derivatives of monosaccharides, which are major components of various carbohydrate materials, were completely separated within 25 min.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Anumula KR (1993) Quantitative monosaccharide analysis of glycoproteins as anthranilyl derivatives by reverse phase HPLC. Glycobiology 3:511–514
Anumula KR (1994) Quantitative determination of monosaccharides in glycoproteins by high performance liquid chromatography with highly sensitive fluorescence detection. Anal Biochem 220:275–283
Bigge JC, Patel TP, Bruce JA, Goulding PN, Charles SM, Parekh RB (1995) Non-selective and efficient fluorescent labeling of glycans using 2-aminobenzamide and anthranilic acid. Anal Biochem 230:229–238
Sato K, Sato K, Okubo A, Yamazaki S (1997) Determination of monosaccharides derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis. Anal Biochem 251:119–121
Sato K, Sato K, Okubo A, Yamazaki S (1998) Optimization of derivatization with 2-Âaminobenzoic acid for determination of Âmonosaccharide composition by capillary electrophoresis. Anal Biochem 262:195–197
He LP, Sato K, Abo M, Okubo A, Yamazaki S (2003) Separation of saccharidesderivatized with 2-aminobenzoic acid by capillary electrophoresis and their structural consideration by nuclear magnetic resonance. Anal Biochem 314:128–134
Chen J, He LP, Abo M, Zhang J, Sato K, Okubo A (2009) Influence of borate complexation on the electrophoretic behavior of 2-AA derivatized saccharides in capillary electrophoresis. Carbohydr Res 344:1141–1145
Hutchins RO (1991) Reduction of C=N to CHNH by metal hydrides. Compr Org Syn 8:36
Borch RF, Bernstein MD, Durst HD (1971) The cyanohydridoborate anion as a selective reducing agent. J Am Chem Soc 93:2897–2904
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Abo, M., He, Lp., Sato, K., Okubo, A. (2013). Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis. In: Volpi, N., Maccari, F. (eds) Capillary Electrophoresis of Biomolecules. Methods in Molecular Biology, vol 984. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-296-4_4
Download citation
DOI: https://doi.org/10.1007/978-1-62703-296-4_4
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-295-7
Online ISBN: 978-1-62703-296-4
eBook Packages: Springer Protocols