Abstract
The use of fluorescent probes can be an easy and quick method to analyze whether or not reactive oxygen species (ROS) are involved in a particular cellular process or the result of a particular drug treatment. ROS activate a variety of cell signaling and death pathways including apoptosis and necrosis (Raha and Robinson. Am J Med Gen 106:62–70, 2001). Here we describe a number of different probes, their specificities, advantages and limitations, as well as useful techniques important for their use.
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Dinnen, R.D., Mao, Y., Fine, R.L. (2013). The Use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_18
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DOI: https://doi.org/10.1007/978-1-62703-287-2_18
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-287-2
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