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The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies

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Lymphoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 971))

Abstract

Chromosomal translocations involving the immunoglobulin (IG) loci are frequently seen in most subtypes of B-cell malignancy and have both diagnostic and prognostic utility. These translocations can be detected in clinical samples by several techniques including metaphase cytogenetics, interphase fluorescent in situ hybridization, and a variety of PCR methods; interphase FISH is the most commonly used clinical method. Although all the common IG translocations have been identified and cloned, new IG translocations continue to be identified in both B-cell leukemia and lymphoma. It remains important to identify the involved target genes since they define novel pathogenetic drivers of disease and may represent novel therapeutic targets. This brief chapter outlines methods of detection of chromosomal translocations involving the IGHJ segments using long distance inverse (LDI) PCR and their application to clinical lymphoma samples.

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Dedication and Acknowledgments

This chapter is dedicated to all those who have worked in my laboratory over the past 20 years and have contributed to the development of LDI-PCR, including Drs Tony Willis, Loraine Karran, Takashi Sonoki, and last but certainly not least, Takashi Akasaka; it has been a privilege to work with you. I gratefully acknowledge generous financial support from the Medical Research Council, Leukemia Lymphoma Research, the Hope Against Cancer. Additional references and offprints of selected articles may be obtained on request.

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Authors and Affiliations

  1. Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK

    Martin J. S. Dyer

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  1. Martin J. S. Dyer
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Correspondence to Martin J. S. Dyer .

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Editors and Affiliations

  1. Medical School, Institute of Cell Biology (Cancer Resear, University of Duisburg-Essen, Virchowstr. 173, Essen, 45122, Germany

    Ralf Küppers

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Dyer, M.J.S. (2013). The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies. In: Küppers, R. (eds) Lymphoma. Methods in Molecular Biology, vol 971. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-269-8_7

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  • DOI: https://doi.org/10.1007/978-1-62703-269-8_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-268-1

  • Online ISBN: 978-1-62703-269-8

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