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Regulation of Dopamine Transporter Expression by Neuronal Activity

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Dopamine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 964))

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Abstract

Actions of extracellular dopamine released in the central nervous system are primarily terminated by the dopamine transporter. This protein is also a target for therapeutic and abused psychostimulant drugs. Different methods used to study dopamine transporter function, its expression, and intracellular signaling in neurons are described in this chapter. Function of the dopamine transporter in mesencephalic primary cultures can be measured by dopamine uptake assay. Expression of the transporter protein and mRNA are analyzed by western blots and quantitative RT-PCR, respectively. Finally, methods to study neuronal activity-dependent changes in Ca2+⁄calmodulin-dependent protein (CaM) kinase activity in dopamine neurons are described.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, Medical College of Georgia, Augusta, GA, USA

    Shalini Padmanabhan & Balakrishna M. Prasad

  2. General Surgery, Dwight D. Eisenhower Army Medical Center, Fort Gordon, GA, USA

    Thach Pham

  3. Clinical Investigation, Dwight D. Eisenhower Army Medical Center, Fort Gordon, GA, USA

    Balakrishna M. Prasad

Authors
  1. Shalini Padmanabhan
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  2. Thach Pham
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  3. Balakrishna M. Prasad
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Corresponding author

Correspondence to Balakrishna M. Prasad .

Editor information

Editors and Affiliations

  1. Krasnow Institute for Advanced Study, Dept. of Molecular Neuroscience, George Mason University, University Drive 4400, Fairfax, 22030, Virginia, USA

    Nadine Kabbani

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Padmanabhan, S., Pham, T., Prasad, B.M. (2013). Regulation of Dopamine Transporter Expression by Neuronal Activity. In: Kabbani, N. (eds) Dopamine. Methods in Molecular Biology, vol 964. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-251-3_14

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  • DOI: https://doi.org/10.1007/978-1-62703-251-3_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-250-6

  • Online ISBN: 978-1-62703-251-3

  • eBook Packages: Springer Protocols

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