Abstract
Cellular senescence, which can be defined as a stress response preventing the propagation of cells that have accumulated potentially oncogenic alterations, is invariably associated with a permanent cell cycle arrest. Such an irreversible blockage is mainly mediated by the persistent upregulation of one or more cyclin-dependent kinase inhibitors (CKIs), including (though not limited to) p16INK4A and p21CIP1 and p27KIP1. CKIs operate by binding to cyclin-dependent kinases (CDKs), de facto inhibiting their enzymatic activity. Here, we provide an immunoblotting-based method for the detection and quantification of CKIs in vitro and ex vivo, together with a set of guidelines for the interpretation of results.
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Acknowledgments
OK, LG and GK are especially grateful to Mr. Sundaramoorthy Balasubramanian, Senior Project Manager at SPi Global for assistance with the production of this book. GK is supported by the Ligue Nationale contre le Cancer (Equipe labellisée), Agence Nationale de la Recherche, Cancéropôle Ile-de-France, Fondation pour la Recherche Médicale, Institut National du Cancer, European Commission (Active p53, Apo-Sys, RIGHT, TransDeath, ChemoRes, ApopTrain), Fondation pour la Recherche Médicale, and the LabEx Immuno-Oncology.
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Kepp, O. et al. (2013). Quantification of Cell Cycle-Arresting Proteins. In: Galluzzi, L., Vitale, I., Kepp, O., Kroemer, G. (eds) Cell Senescence. Methods in Molecular Biology, vol 965. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-239-1_7
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DOI: https://doi.org/10.1007/978-1-62703-239-1_7
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