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Monitoring Extracellular Monoamines with In Vivo Microdialysis in Awake Rats: A Practical Approach

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Microdialysis Techniques in Neuroscience

Part of the book series: Neuromethods ((NM,volume 75))

Abstract

The microdialysis technique for the measurement of brain extracellular levels of monoamines has become very popular over the last 2–3 decades, particularly in laboratories involved in neuropharmacology studies. Nevertheless, microdialysis of monoamines is a challenging technique for several reasons and to get reliable results it is necessary to develop sufficient skill and be ready to solve technical and analytical problems that may occur. This chapter is intended to help obtain reliable and reproducible results by describing in detail practical aspects of probe construction and implantation into the rat brain and measurement of noradrenaline, dopamine, and serotonin with HPLC coupled to electrochemical detection, set up, optimized, and validated in this author’s laboratory.

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Acknowledgements

I wish to thank all my coworkers that have contributed to set up, optimize, and validate the microdialysis technique applied to monoamine measurement in the rat, mouse, and gerbil brain as currently used in this author’s laboratory. This Chapter is dedicated to the memory of my mentor, Dr. R. Samanin, who passed away in 2001.

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Authors and Affiliations

  1. Laboratory of Neurochemistry and Behavior, Department of Neuroscience, Istituto di Ricerche Farmacologiche “Mario Negri”, Milan, Italy

    Roberto W. Invernizzi

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  1. Roberto W. Invernizzi
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Corresponding author

Correspondence to Roberto W. Invernizzi .

Editor information

Editors and Affiliations

  1. , Dept. of Physiology & Biochemistry, University of Malta, Biomedical Sciences Building, Msida MSD, 2080, Malta

    Giuseppe Di Giovanni

  2. Consorzio "Mario Negri" Sud, Via Nazionale, Santa Maria Imbaro, 66030, Chieti, Italy

    Vincenzo Di Matteo

Appendix

Appendix

List of main suppliers and Web page address

Supplier

Product

Web page address

Beckman

Collection vials

www.beckmancoulter.com

Carlo Erba Reagenti

Chemicals

www.carloerbareagents.com

Clark Electromedical

Tungsten wires

www.warneronline.com

CMA/Microdialysis

Microinfusion pump

www.microdialysis.se

Coopers Needle Work

Stainless steel tubing

www.finestainlesstube.com

Datalys

Chromatographic software

www.datalys.net

David Kopf Instruments

Stereotaxic

www.kopfinstruments.com

Eicom

Swivels

www.eicomeurope.com

Eppendorf

Tubes

www.eppendorf.com

ESA

Electrochemical detector

www.esainc.com

Fluka

Monoamine standards

www.sigmaaldrich.com

Harvard Apparatus

Microinfusion pump

www.harvardapparatus.com

Hospal

Microdialysis fibers

www.hospal.it

Instech

Swivels

www.instechlab.com

Leica

Stereomicroscope

www.leicaitalia.it

Merck

Chemicals

www.merck-chemicals.com

Microbiotech

FEP tubing

www.microbiotech.se

Millipore

HPLC-grade water

www.millipore.com

Polymicro Technologies

Silica tubing

www.polymicro.com

Portex

PE tubing

www.smithsmedical.com

Schleicher & Schuell

Disc filters

www.schleicher-schuell.com

Shimadzu

HPLC pumps

www.shimadzu.it

Shiseido

HPLC columns

www.shiseido.co.jp

Sorin Biomedica

Microdialysis fibers

www.sorin.com

Spark Holland

Autosamplers

www.sparkholland.com

SSI-LabAlliance

Pulse dampener

www.laballiance.com

Supelco

HPLC columns

www.sigmaaldrich.com

Univentor

Fraction collectors

www.univentor.com

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Invernizzi, R.W. (2013). Monitoring Extracellular Monoamines with In Vivo Microdialysis in Awake Rats: A Practical Approach. In: Di Giovanni, G., Di Matteo, V. (eds) Microdialysis Techniques in Neuroscience. Neuromethods, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-173-8_9

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  • DOI: https://doi.org/10.1007/978-1-62703-173-8_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-172-1

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