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Quantitative In Vivo Microdialysis in Pharmacokinetic Studies

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Microdialysis Techniques in Neuroscience

Part of the book series: Neuromethods ((NM,volume 75))

Abstract

Recent theoretical studies have yielded a more profound knowledge of the properties of recovery (the key parameter in quantitative microdialysis) and have put in evidence important limitations of the usual in vivo calibration methods used in quantitative microdialysis for pharmacokinetic studies. Recovery values obtained by using the more classical methods of calibration (the variation of flow rate perfusion method, the delivery and retrodialysis methods, and the no net flux method) can only be used to accurately convert dialysate drug concentrations into extracellular concentrations, when the drug of interest is in the body under steady-state conditions. Therefore, these in vivo calibration procedures must not be used when the drug studied has to be administered using modalities of administration which do not provide steady-state concentrations (for example, intragastric, subcutaneous, intraperitoneal, or intravenous bolus injections). The dynamic no net flux (DNNF) method, however, can be considered the only in vivo calibration method useful in PK experiments developed under transient conditions, although this calibration procedure has several serious disadvantages. The new modified version of the ultraslow microdialysis (the MetaQuant technique) overcomes many of the limitations of both the classical calibration and the DNNF methods and, therefore, it could be considered a promising tool in pharmacokinetics.

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Abbreviations

C d :

Drug concentration in the dialysate

C ecf :

Drug concentration in the extracellular fluid

C in :

Drug concentration in perfusate

CSF:

Cerebrospinal fluid

DNNF:

Dynamic no net flux method

E :

Extraction fraction, extraction efficiency or recovery

ECF:

Extracellular fluid

MD:

Microdialysis

NNF:

No net flux

PK:

Pharmacokinetics

Q :

Perfusion flow rate

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Authors and Affiliations

  1. Department of Pharmacy and Pharmaceutical Technology, University of Valencia, Valencia, Spain

    Teodoro Zornoza, María José Cano-Cebrián, Ana Polache & Luis Granero

Authors
  1. Teodoro Zornoza
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  2. María José Cano-Cebrián
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  3. Ana Polache
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  4. Luis Granero
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Corresponding author

Correspondence to Luis Granero .

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Editors and Affiliations

  1. , Dept. of Physiology & Biochemistry, University of Malta, Biomedical Sciences Building, Msida MSD, 2080, Malta

    Giuseppe Di Giovanni

  2. Consorzio "Mario Negri" Sud, Via Nazionale, Santa Maria Imbaro, 66030, Chieti, Italy

    Vincenzo Di Matteo

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Zornoza, T., Cano-Cebrián, M.J., Polache, A., Granero, L. (2013). Quantitative In Vivo Microdialysis in Pharmacokinetic Studies. In: Di Giovanni, G., Di Matteo, V. (eds) Microdialysis Techniques in Neuroscience. Neuromethods, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-173-8_6

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  • DOI: https://doi.org/10.1007/978-1-62703-173-8_6

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  • Publisher Name: Humana Press, Totowa, NJ

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