Abstract
In vivo overproduction of tRNA chimeras yields an RNA insert within a tRNA scaffold. For some applications, it may be necessary to discard the scaffold. Here we present a protocol for selective cleavage of the RNA of interest from the tRNA scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
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Ponchon, L., Beauvais, G., Nonin-Lecomte, S., Dardel, F. (2013). Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. In: Conn, G. (eds) Recombinant and In Vitro RNA Synthesis. Methods in Molecular Biology, vol 941. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-113-4_2
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DOI: https://doi.org/10.1007/978-1-62703-113-4_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-112-7
Online ISBN: 978-1-62703-113-4
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